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Nucleic Acids Research, Vol 26, Issue 14 3449-3450, Copyright © 1998 by Oxford University Press

ARTICLES

Chemical mapping of co-existing RNA structures

AR Schroder, T Baumstark and D Riesner
Institut fur Physikalische Biologie, Heinrich-Heine-Universitat Dusseldorf, D-40225 Dusseldorf, Germany.

In many cases RNA can assume co-existing or meta-stable structures preventing structure determination by chemical mapping. A novel method is described, by which RNA is modified with dimethyl sulphate without shifting the distribution of different structures. The different structures are then separated in native gel electrophoresis, and structure determination by primer extension can be carried out separately for each structure.
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