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Nucleic Acids Research, Vol 26, Issue 14 3451-3452, Copyright © 1998 by Oxford University Press

ARTICLES

Solid-phase cDNA library construction, a versatile approach

T Roeder
University of Hamburg, Zoological Institute, Department of Neurophysiology, Martin-Luther-King-Platz 3,D-20146 Hamburg, Germany. roeder@zoologie.uni-hamburg.de

A rapid and versatile method for cDNA library construction was developed. It is based on conventional cDNA library synthesis including all enzymatic steps usually required, but is performed on a solid support. The cDNA is immobilised via a biotin residue to streptavidin coupled magnetic beads, which allows rapid and easy to perform changes of buffers and enzymes. Therefore, it combines speed (library construction within a single day) with high quality libraries, making it ideally suited for most purposes.
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