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Nucleic Acids Research, Vol 26, Issue 15 3550-3554, Copyright © 1998 by Oxford University Press

ARTICLES

Transcription of RNA templates by T7 RNA polymerase

N Arnaud-Barbe, V Cheynet-Sauvion, G Oriol, B Mandrand and F Mallet
Unite Mixte 103 CNRS-bioMerieux, Ecole Normale Superieure de Lyon, 46 allee d'Italie, 69364 Lyon, Cedex 07, France.

Although highly specialized, T7 RNA polymerase seems to possess a large range of DNA- and RNA-dependent properties. To study such flexibility, we determined the ability of T7 RNA polymerase to transcribe chimeric DNA-RNA and RNA templates following initiation at a double stranded DNA promoter. We have found that T7 RNA polymerase is able to initiate on RNA templates, was processive, and was able to use templates containing RNA-RNA duplexes under standard transcription conditions. Implications of remnants of such RNA-dependent activities for T7 DNA-dependent polymerase are discussed.
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