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Nucleic Acids Research, Vol 26, Issue 15 3599-3607, Copyright © 1998 by Oxford University Press

ARTICLES

Neighboring base damage induced by permanganate oxidation of 8- oxoguanine in DNA

S Koizume, H Inoue, H Kamiya and E Ohtsuka
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi- 6, Kita-ku, Sapporo 060-0812, Japan.

We found that single-stranded DNA oligomers containing a 7, 8-dihydro-8- oxoguanine (8-oxo-G) residue have high reactivity toward KMnO4; the oxidation of 8-oxo-G induces damage to the neighboring nucleotide residues. This paper describes the novel reaction in detail, including experiments that demonstrate the mechanism involved in the induction of DNA damage. The results using DNAs of various base compositions indicated that damaged G, T and C (but not A) sites caused strand scissions after hot piperidine treatment and that the damage around the 8-oxo-G occurred at G sites in both single and double strands with high frequency. The latter substrates were less sensitive to damage. Further, kinetic studies of the KMnO4reaction of single-stranded oligomers suggested that thereactivity of the DNA bases at the site 5'- adjacent to the 8-oxo-G was in the order G >A >T, C. This preference correlates with the electron donating abilities of the bases. In addition, we found that the DNA damage at the G site, which was connected with the 8-oxo-G by a long abasic chain, was inhibited in the above order by the addition of dG, dA or dC. On the other hand, the damage reactions proceeded even after the addition of scavengers for active oxygen species. This study suggests the involvement of a redox process in the unique DNA damage initiated by the oxidation of the 8- oxo-G.
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