Nucleic Acids Research, Vol 26, Issue 16 3687-3693, Copyright © 1998 by Oxford University Press
L Zhang, U Sankar, DJ Lampe, HM Robertson and FL Graham
Mariner transposons belong to the mariner /Tc1 superfamily of class II,
DNA-mediated elements. One of these transposons, Himar1 , isolated from the
horn fly, is independent of host-specific factors that would limit transfer
between different species, making it an ideal candidate for gene transfer
technology development. To determine the activity of Himar1 transposase in
mammalian cells, we introduced the Himar1 transposase gene into an
adenovirus (Ad) vector under control of the phage T7 RNA polymerase
promoter. Mammalian cells infected with the Ad vector carrying the Himar1
gene efficiently expressed the Himar1 transposase in the presence of T7
polymerase. In in vitro inter-plasmid transposition reactions, Himar1
transposase expressed by the Ad vector mediated precise cut-and-paste
transposition and resulted in a characteristic duplication of TA at the
integration site of the target plasmid. Further studies showed that this
transposase was capable of catalyzing transposition between twoplasmids
co-transfected into 293T7pol cells, which express T7 RNA polymerase.
Combining the integration capability of mariner transposons with the
transduction efficiency of Ad vectors is expected to provide a powerful
tool for introducing transgenes into the host chromosome.
ARTICLES
The Himar1 mariner transposase cloned in a recombinant adenovirus vector is functional in mammalian cells
Department of Biology and Department of Pathology, McMaster University, Hamilton, Ontario L8S 4K1, Canada.
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