Nucleic Acids Research, Vol 26, Issue 16 3739-3745, Copyright © 1998 by Oxford University Press
I Treich, L Ho and M Carlson
The RSC complex of Saccharomyces cerevisiae is closely related to the
SWI/SNF complex. Both complexes are involved in remodeling chromatin
structure and they share conserved components. The RSC proteins Sth1,
Rsc8/Swh3, Sfh1 and Rsc6 are homologs of the SWI/SNF proteins Swi2/Snf2,
Swi3, Snf5 and Swp73 respectively. To investigate the RSC complex, we
isolated a temperature-sensitive swh3 allele. A screen for multicopy
suppressors yielded plasmids carrying the RSC6 and MAK31 loci. RSC6 also
suppressed the formamide sensitivity of a strain with a C-terminal
truncation of SWH3 . We show that Swh3 and Rsc6 fusion proteins interact in
the two-hybrid system and that the swh3-ts mutation impairs this
interaction. Finally, bacterially produced Swh3 and Rsc6 fusion proteins
interact in vitro , supporting the genetic evidence for direct interaction
between Swh3 and Rsc6 in vivo . We have previously shown that Swh3 also
interacts with Sth1. These findings, together with the conservation of
these proteins in the SWI/SNF complex and in mammalian SWI/SNF-related
complexes, strongly suggest that these proteins form a structural core for
the complex.
ARTICLES
Direct interaction between Rsc6 and Rsc8/Swh3,two proteins that are conserved in SWI/SNF-related complexes
Department of Genetics and Development and Institute of Cancer Research and Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
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