Nucleic Acids Research, Vol 26, Issue 16 3784-3788, Copyright © 1998 by Oxford University Press
MO Gold and AP Rice
During transcription of mRNA genes, there is a correlation between the
phosphorylation state of the C-terminal domain (CTD) of the large subunit
of RNA polymerase II (RNAP II) and the ability of the RNAP II complex to
processively transcribe the gene. To examine the involvement of CTD
phosphorylation in modulation of RNAP II function, we have analyzed the
ability of a known CTD kinase, human Cdk8, to modulate HIV- 1 LTR-driven
gene expression upon directed targeting to a promoter- proximal nascent RNA
element. The results indicated that Cdk8, when localized to an RNA element,
activates gene expression in a catalysis- dependent manner. Also, Cdk8
targeted to RNA was observed to act in a synergystic manner with
DNA-targeted Sp1 but not with DNA-targeted HIV- 1 Tat, suggesting that
RNA-targeted Cdk8 acts on similar rate limiting post-initiation events as
Tat. As recent observations suggest that Tat/TAR-mediated transcription of
the proviral genome of HIV depends on specific phosphorylation of RNAP II
in its CTD by the Tat-associated kinase (TAK/p-TEFb/Cdk9), our results
indicate that Cdk8 shares with Cdk9 the ability to modulate transcription
upon targeting to a nascent RNA element.
ARTICLES
Targeting of CDK8 to a promoter-proximal RNA element demonstrates catalysis-dependent activation of gene expression
Division of Molecular Virology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA.
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