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Nucleic Acids Research, Vol 26, Issue 17 3940-3943, Copyright © 1998 by Oxford University Press

ARTICLES

Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4

D Ramaiah, T Koch, H Orum and GB Schuster
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

The specific reaction of potassium permanganate with thymine in single- stranded DNA was employed to analyze thymine [2+2] dimer repair in DNA and in DNA/peptide nucleic acid hybrid duplexes. This simple and highly sensitive chemical assay is convenient for monitoring repair of thymine dimers in oligonucleotides.
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