Nucleic Acids Research, Vol 26, Issue 17 4019-4025, Copyright © 1998 by Oxford University Press
KJ Dej, T Gerasimova, VG Corces and JD Boeke
The Drosophila retroelement gypsy has a number of unusual features
including an unusual LTR terminal sequence and an apparent target sequence
preference. The ovo locus is a known hotspot for gypsy insertion. We
examined the target sequence preference of gypsy within ovo by isolating 26
new insertions and sequencing the gypsy/ovo junctions. Insertions were
found at multiple sites within the ovo locus. The insertions clustered
within an approximately 150 bp region in the non-translated region of the
ovo beta transcript, with most insertions falling within the first intron.
There were seven sites of insertion within this region and these mostly
conform to the consensus sequence YRYRYR (where Y = pyrimidine and R =
purine). However, this target sequence is at best necessary but not
sufficient to specify a hotspot, as there were several other sequences
conforming to this consensus in the ovo locus that were not hit. The
results indicate that gypsy may have a higher degree of target specificity
than most infectious LTR retroelements.
ARTICLES
A hotspot for the Drosophila gypsy retroelement in the ovo locus
Department of Biology, Johns Hopkins University and Department of Molecular Biology and Genetics,Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Labrador, K. Sha, A. Li, and V. G. Corces Insulator and Ovo Proteins Determine the Frequency and Specificity of Insertion of the gypsy Retrotransposon in Drosophila melanogaster Genetics, November 1, 2008; 180(3): 1367 - 1378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Glasscock and M. A. Tanouye Drosophila couch potato Mutants Exhibit Complex Neurological Abnormalities Including Epilepsy Phenotypes Genetics, April 1, 2005; 169(4): 2137 - 2149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Copeland, P. J. Brindley, O. Heyers, S. F. Michael, D. A. Johnston, D. L. Williams, A. C. Ivens, and B. H. Kalinna Boudicca, a Retrovirus-Like Long Terminal Repeat Retrotransposon from the Genome of the Human Blood Fluke Schistosoma mansoni J. Virol., June 1, 2003; 77(11): 6153 - 6166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Labrador and V. G. Corces Protein Determinants of Insertional Specificity for the Drosophila Gypsy Retrovirus Genetics, July 1, 2001; 158(3): 1101 - 1110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Lu and B Oliver Drosophila OVO regulates ovarian tumor transcription by binding unusually near the transcription start site Development, January 5, 2001; 128(9): 1671 - 1686. [Abstract] [PDF]
|
|
|
|
 |
|
 N. J. Bowen and J. F. McDonald Genomic Analysis of Caenorhabditis elegans Reveals Ancient Families of Retroviral-like Elements Genome Res., October 1, 1999; 9(10): 924 - 935. [Abstract] [Full Text]
|
|
|
|
|
|
P. Leblanc, B. Dastugue, and C. Vaury The Integration Machinery of ZAM, a Retroelement from Drosophila melanogaster, Acts as a Sequence-Specific Endonuclease J. Virol., August 1, 1999; 73(8): 7061 - 7064. [Abstract] [Full Text]
|
|
|
|
|
|
H. S. Malik and T. H. Eickbush Modular Evolution of the Integrase Domain in the Ty3/Gypsy Class of LTR Retrotransposons J. Virol., June 1, 1999; 73(6): 5186 - 5190. [Abstract] [Full Text]
|
|