Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (116K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (8)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Marx, A.
Right arrow Articles by Giese, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marx, A.
Right arrow Articles by Giese, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 26, Issue 17 4063-4067, Copyright © 1998 by Oxford University Press

ARTICLES

4'-Acylated thymidine 5'-triphosphates: a tool to increase selectivity towards HIV-1 reverse transcriptase

A Marx, M Amacker, M Stucki, U Hubscher, TA Bickle and B Giese
Department of Chemistry, University of Basel, St Johanns-Ring 19, CH- 4056 Basel, Switzerland.

4'-Acylated thymidines represent a new class of DNA chain terminators, since they have been shown to act as post-incorporation chain- terminating nucleotides despite the presence of a free 3'-hydroxyl group. Here, we describe the action of the 4'-acetyl- (MeTTP) and 4'- propanoylthymidine 5'-triphosphate (EtTTP) on HIV-1 reverse transcriptase in RNA- and DNA-dependent DNA synthesis and on DNA synthesis catalyzed by the cellular DNA polymerases alpha, beta, delta and epsilon. MeTTP exhibits a high selectivity towards HIV-1 reverse transcriptase. By the use of the bulkier propanoyl group as the 4'- substituent of the nucleoside 5'-triphosphate, selectivity towards HIV- 1 reverse transcriptase could be increased without affecting substrate efficiency. Thus, 4'-modifications may serve as a tool to increase selectivity towards HIV-1 reverse transcriptase.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
B. A. Mulder, S. Anaya, P. Yu, K. W. Lee, A. Nguyen, J. Murphy, R. Willson, J. M. Briggs, X. Gao, and S. H. Hardin
Nucleotide modification at the {gamma}-phosphate leads to the improved fidelity of HIV-1 reverse transcriptase
Nucleic Acids Res., September 1, 2005; 33(15): 4865 - 4873.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. Kempeneers, M. Renders, M. Froeyen, and P. Herdewijn
Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization
Nucleic Acids Res., July 12, 2005; 33(12): 3828 - 3836.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Vastmans, M. Froeyen, L. Kerremans, S. Pochet, and P. Herdewijn
Reverse transcriptase incorporation of 1,5-anhydrohexitol nucleotides
Nucleic Acids Res., August 1, 2001; 29(15): 3154 - 3163.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.