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Nucleic Acids Research, Vol 26, Issue 18 4116-4120, Copyright © 1998 by Oxford University Press

ARTICLES

Sequence specificity of the hammerhead ribozyme revisited; the NHH rule

AR Kore, NK Vaish, U Kutzke and F Eckstein
Max-Planck-Institut fur Experimentelle Medizin, Hermann-Rein-Strasse 3, D-37075 Gottingen, Germany.

The sequence specificity of hammerhead ribozyme cleavage has been re- evaluated with respect to the NUH rule. Contrary to previous reports it was found that substrates with GAC triplets were also cleaved. This was established in three different sequence contexts. The rate of cleavage under single turnover conditions was between 3 and 7% that of cleavage 3' of GUC. Specificity of cleavage of substrates containing a central A in the cleavable triplet can be described as NAH, where N can be any nucleotide and H any nucleotide but G. As cleavage 3' of NCH triplets has recently been described, the NUH rule can be reformulated to NHH.
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