Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (155K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (45)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Tang, J.
Right arrow Articles by Breaker, R. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tang, J.
Right arrow Articles by Breaker, R. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 26, Issue 18 4214-4221, Copyright © 1998 by Oxford University Press

ARTICLES

Mechanism for allosteric inhibition of an ATP-sensitive ribozyme

J Tang and RR Breaker
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8103, USA.

We report the structural basis for the modulation of an ATP-sensitive ribozyme that was engineered by modular rational design. This allosteric ribozyme is composed of two independently functioning domains, one a receptor for ATP and the other a self-cleaving ribozyme. When fused in the appropriate fashion, the conjoined aptamer-ribozyme construct functions as an allosteric ribozyme that is inhibited in the presence of ATP. The aptamer domain remains conformationally heterogeneous in the absence of ATP, but folds into a distinct structure upon ligand binding. This ATP-induced conformational change causes a reduction in catalytic activity of the adjacent ribozyme domain due to steric interference between the aptamer and ribozyme tertiary structures. This mechanism for structural and functional modulation of nucleic acids is one of several possible mechanisms by which the function of ribozymes could be specifically controlled by small effector molecules.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. J. Tabor, M. Levy, and A. D. Ellington
Deoxyribozymes that recode sequence information.
Nucleic Acids Res., January 1, 2006; 34(8): 2166 - 2172.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Cho, J.-E. Kim, B.-R. Lee, J.-H. Kim, and B.-G. Kim
Bis-aptazyme sensors for hepatitis C virus replicase and helicase without blank signal
Nucleic Acids Res., November 27, 2005; 33(20): e177 - e177.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
C. C. Deocaris, S. C. Kaul, K. Taira, and R. Wadhwa
Emerging Technologies: Trendy RNA Tools for Aging Research
J. Gerontol. A Biol. Sci. Med. Sci., August 1, 2004; 59(8): B771 - B783.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. K. SILVERMAN
Rube Goldberg goes (ribo)nuclear? Molecular switches and sensors made from RNA
RNA, April 1, 2003; 9(4): 377 - 383.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Kertsburg and G. A. Soukup
A versatile communication module for controlling RNA folding and catalysis
Nucleic Acids Res., November 1, 2002; 30(21): 4599 - 4606.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. M. Jose, G. A. Soukup, and R. R. Breaker
Cooperative binding of effectors by an allosteric ribozyme
Nucleic Acids Res., April 1, 2001; 29(7): 1631 - 1637.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. A. Soukup and R. R. Breaker
Engineering precision RNA molecular switches
PNAS, March 30, 1999; 96(7): 3584 - 3589.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.