Nucleic Acids Research, Vol 26, Issue 18 4267-4273, Copyright © 1998 by Oxford University Press
N Nishiya, H Sabe, K Nose and M Shibanuma
hic-5 protein is a member of the LIM protein family, containing four LIM
domains in its C-terminal region. It is mainly localized in focal adhesions
and shows striking similarity to paxillin in its LIM domains, although the
function of these LIM domains has remained elusive. In the present study,
we found that full-length and the C-terminal half of hic- 5 protein,
including four LIM domains, bound to DNA in a zinc-dependent manner in
vitro . Mouse genomic fragments that specifically bound to the hic-5
protein were isolated by successive rounds of hic-5 protein- DNA complex
immunoprecipitation and PCR amplification. Seven independent clones were
isolated, which contained high amounts of G+A and/or a long A/T tract. A
DNA binding protein blot assay revealed the specificity of the interaction
between hic-5 protein and the DNA fragment. Using a series of truncated
forms of the hic-5 LIM domains, each of the four LIM domains was found to
contribute to DNA binding in a distinctive manner.
ARTICLES
The LIM domains of hic-5 protein recognize specific DNA fragments in a zinc-dependent manner in vitro
Department of Microbiology, Showa University School of Pharmaceutical Sciences, Hatanodai 1-5-8, Shinagawa-ku, Tokyo, Japan and Osaka Bioscience Institute, Suita, Osaka, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Mori, M. Asakawa, M. Hayashi, M. Imura, T. Ohki, E. Hirao, J.-r. Kim-Kaneyama, K. Nose, and M. Shibanuma Oligomerizing Potential of a Focal Adhesion LIM Protein Hic-5 Organizing a Nuclear-Cytoplasmic Shuttling Complex J. Biol. Chem., August 4, 2006; 281(31): 22048 - 22061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Ghogomu, S. van Venrooy, M. Ritthaler, D. Wedlich, and D. Gradl HIC-5 Is a Novel Repressor of Lymphoid Enhancer Factor/T-cell Factor-driven Transcription J. Biol. Chem., January 20, 2006; 281(3): 1755 - 1764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, K. Song, T. L. Sponseller, and D. Danielpour Novel Function of Androgen Receptor-associated Protein 55/Hic-5 as a Negative Regulator of Smad3 Signaling J. Biol. Chem., February 18, 2005; 280(7): 5154 - 5162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R.M.O. Crombez, E. M.R. Vanoirbeek, W. J.M. Van de Ven, and M. M.R. Petit Transactivation Functions of the Tumor-Specific HMGA2/LPP Fusion Protein Are Augmented by Wild-Type HMGA2 Mol. Cancer Res., February 1, 2005; 3(2): 63 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Brown and C. E. Turner Paxillin: Adapting to Change Physiol Rev, October 1, 2004; 84(4): 1315 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Rahman, H. Miyamoto, and C. Chang Androgen Receptor Coregulators in Prostate Cancer: Mechanisms and Clinical Implications Clin. Cancer Res., April 1, 2004; 10(7): 2208 - 2219. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Shibanuma, J.-r. Kim-Kaneyama, K. Ishino, N. Sakamoto, T. Hishiki, K. Yamaguchi, K. Mori, J.-i. Mashimo, and K. Nose Hic-5 Communicates between Focal Adhesions and the Nucleus through Oxidant-Sensitive Nuclear Export Signal Mol. Biol. Cell, March 1, 2003; 14(3): 1158 - 1171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Heinlein and C. Chang Androgen Receptor (AR) Coregulators: An Overview Endocr. Rev., April 1, 2002; 23(2): 175 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Gay, I. Anglade, Z. Gong, and G. Salbert The LIM/Homeodomain Protein Islet-1 Modulates Estrogen Receptor Functions Mol. Endocrinol., October 1, 2000; 14(10): 1627 - 1648. [Abstract] [Full Text]
|
|
|
|
|
|
L. Yang, J. Guerrero, H. Hong, D. B. DeFranco, and M. R. Stallcup Interaction of the tau 2 Transcriptional Activation Domain of Glucocorticoid Receptor with a Novel Steroid Receptor Coactivator, Hic-5, Which Localizes to Both Focal Adhesions and the Nuclear Matrix Mol. Biol. Cell, June 1, 2000; 11(6): 2007 - 2018. [Abstract] [Full Text]
|
|
|
|
|
|
M. M. R. Petit, J. Fradelizi, R. M. Golsteyn, T. A.Y. Ayoubi, B. Menichi, D. Louvard, W. J. M. Van de Ven, and E. Friederich LPP, an Actin Cytoskeleton Protein Related to Zyxin, Harbors a Nuclear Export Signal and Transcriptional Activation Capacity Mol. Biol. Cell, January 1, 2000; 11(1): 117 - 129. [Abstract] [Full Text]
|
|
|
|
|
|
N. Nishiya, Y. Iwabuchi, M. Shibanuma, J.-F. Cote, M. L. Tremblay, and K. Nose Hic-5, a Paxillin Homologue, Binds to the Protein-tyrosine Phosphatase PEST (PTP-PEST) through Its LIM 3 Domain J. Biol. Chem., April 2, 1999; 274(14): 9847 - 9853. [Abstract] [Full Text] [PDF]
|
|