Nucleic Acids Research, Vol 26, Issue 19 4324-4331, Copyright © 1998 by Oxford University Press
S Oettel, I Kober and KH Seifart
Human TFIIIC1, a basal transcription factor essentially required for
expression of all pol III genes, exerts its function without primarily
binding to DNA. We report here the purification of a termination site
binding activity (TBA) which was initially described to be contained in
fractions designated as TFIIIC0. TBA specifically and strongly binds to the
termination region of pol III genes with internal promoters and can be
completely separated from TFIIIC1and a TFIIIC1related activity
(TFIIIC1-like), proving that DNA-binding of TBA is independent of these
latter activites. Although TBA is not essentially required for, it strongly
stimulates pol III transcription from intragenic promoters. This
stimulation strictly depends on the presence of TFIIIC1and is not observed
in conjunction with TFIIIC1-like. We further present the identification of
a novel activity, TFIIIU, which is also contained in crude fractions of
TFIIIC0. TFIIIU can be separated from TBA by further purification and is
essentially involved in transcription of the mammalian U6 gene. TFIIIU
cannot be substituted for by any of the established U6 transcription
factors and thus represents a novel U6 transcription factor.
ARTICLES
The activity binding to the termination region of several pol III genes represents a separate entity and is distinct from a novel component enhancing U6 snRNA transcription
Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Lahnstrasse 3, D-35037 Marburg, Germany.
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