Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (486K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gravel, A.
Right arrow Articles by Roy, P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gravel, A.
Right arrow Articles by Roy, P. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 26, Issue 19 4347-4355, Copyright © 1998 by Oxford University Press

ARTICLES

DNA complexes obtained with the integron integrase IntI1 at the attI1 site

A Gravel, B Fournier and PH Roy
Centre de Recherche en Infectiologie, Centre Hospitalier de l'Universite Laval and Departement de Biochimie,Faculte des Sciences et de Genie, Universite Laval, Sainte-Foy, Quebec G1V 4G2, Canada.

Integrons are genetic elements that are able to capture genes by a site- specific recombination mechanism. Integrons contain a gene coding for a lambda-like integrase that carries out site-specific recombination by interacting with two different target sites; the attI site and the palindromic sequence attC (59 base element). Cassette integrations usually involve the attI site, while cassette excisions use attC . Therefore, the integrase should bind both sites to cleave DNA and perform site-specific recombination reactions. We have used purified maltose-binding protein fused with the integrase (MBP-IntI1) and native IntI1 protein and gel retardation assays with fragments containing the complete and partial attI1 site to show formation of four complexes in this region. Chemical modification of specific nucleotides within the attI1 site was used to investigate their interference with binding of the integrase protein. We attribute IntI1 specific binding to four regions in the attI1 site and a GTTA consensus sequence is found in three of the four regions. Interference by modified guanine and thymine residues in the DNA major groove and adenine residues in the minor groove were observed, indicating that the integrase interacts with both sides of the helix. Binding of IntI1 to attC is also discussed.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
M. S. Ramirez, T. R. Parenteau, D. Centron, and M. E. Tolmasky
Functional characterization of Tn1331 gene cassettes
J. Antimicrob. Chemother., October 1, 2008; 62(4): 669 - 673.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Demarre, C. Frumerie, D. N. Gopaul, and D. Mazel
Identification of key structural determinants of the IntI1 integron integrase that influence attC x attI1 recombination efficiency
Nucleic Acids Res., October 8, 2007; 35(19): 6475 - 6489.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Johansson, M. Kamali-Moghaddam, and L. Sundstrom
Integron integrase binds to bulged hairpin DNA
Nucleic Acids Res., August 2, 2004; 32(13): 4033 - 4043.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
C. M. Collis and R. M. Hall
Comparison of the structure-activity relationships of the integron-associated recombination sites attI3 and attI1 reveals common features
Microbiology, May 1, 2004; 150(5): 1591 - 1601.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
A. Reyes, H. Bello, M. Dominguez, S. Mella, R. Zemelman, and G. Gonzalez
Prevalence and types of class 1 integrons in aminoglycoside-resistant Enterobacteriaceae from several Chilean hospitals
J. Antimicrob. Chemother., February 1, 2003; 51(2): 317 - 321.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
S. R. Partridge, C. M. Collis, and R. M. Hall
Class 1 Integron Containing a New Gene Cassette, aadA10, Associated with Tn1404 from R151
Antimicrob. Agents Chemother., August 1, 2002; 46(8): 2400 - 2408.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. M. Collis, M.-J. Kim, S. R. Partridge, H. W. Stokes, and R. M. Hall
Characterization of the Class 3 Integron and the Site-Specific Recombination System It Determines
J. Bacteriol., June 1, 2002; 184(11): 3017 - 3026.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
N. Messier and P. H. Roy
Integron Integrases Possess a Unique Additional Domain Necessary for Activity
J. Bacteriol., November 15, 2001; 183(22): 6699 - 6706.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. M. Collis, G. D. Recchia, M.-J. Kim, H. W. Stokes, and R. M. Hall
Efficiency of Recombination Reactions Catalyzed by Class 1 Integron Integrase IntI1
J. Bacteriol., April 15, 2001; 183(8): 2535 - 2542.
[Abstract] [Full Text]

Home page
 Antimicrob. Agents Chemother.Home page
C. Goldstein, M. D. Lee, S. Sanchez, C. Hudson, B. Phillips, B. Register, M. Grady, C. Liebert, A. O. Summers, D. G. White, et al.
Incidence of Class 1 and 2 Integrases in Clinical and Commensal Bacteria from Livestock, Companion Animals, and Exotics
Antimicrob. Agents Chemother., March 1, 2001; 45(3): 723 - 726.
[Abstract] [Full Text]

Home page
 MicrobiologyHome page
S. R. Partridge, G. D. Recchia, C. Scaramuzzi, C. M. Collis, H. W. Stokes, and R. M. Hall
Definition of the attI1 site of class 1 integrons
Microbiology, November 1, 2000; 146(11): 2855 - 2864.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
B. Fournier, R. Aras, and D. C. Hooper
Expression of the Multidrug Resistance Transporter NorA from Staphylococcus aureus Is Modified by a Two-Component Regulatory System
J. Bacteriol., February 1, 2000; 182(3): 664 - 671.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
M. V. Francia, J. C. Zabala, F. de la Cruz, and J. M. García Lobo
The IntI1 Integron Integrase Preferentially Binds Single-Stranded DNA of the attC Site
J. Bacteriol., November 1, 1999; 181(21): 6844 - 6849.
[Abstract] [Full Text]

Home page
 Microbiol. Mol. Biol. Rev.Home page
C. A. Liebert, R. M. Hall, and A. O. Summers
Transposon Tn21, Flagship of the Floating Genome
Microbiol. Mol. Biol. Rev., September 1, 1999; 63(3): 507 - 522.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.