Nucleic Acids Research, Vol 26, Issue 19 4374-4381, Copyright © 1998 by Oxford University Press
A Soma and H Himeno
Prokaryotes have three amino acid-specific class II tRNAs that possess a
characteristic long variable arm, tRNASer, tRNALeuand tRNATyr, while
eukaryotes have only two, tRNASerand tRNALeu. Because of such a
phylogenetic divergence in the composition of tRNA, the class II tRNA
system is a good candidate for studying how the tRNA recognition manner has
evolved in association with the evolution of tRNA. We report here a
cross-species aminoacylation study of the class II tRNAs, showing the
unilateral aminoacylation specificity between Escherichia coli and a yeast,
Saccharomyces cerevisiae. Both SerRS and LeuRS from E.coli were unable to
aminoacylate yeast class II tRNAs; in contrast, the yeast counterparts were
able to aminoacylate E.coli class II tRNAs. Yeast seryl-tRNA synthetase was
able to aminoacylate not only E.coli tRNASerbut also tRNALeuand tRNATyr,
and yeast LeuRS was able to aminoacylate not only E.coli tRNALeubut also
tRNATyr. These results indicate that the recognition manner of class II
tRNA, especially the discrimination strategy of each aminoacyl-tRNA
synthetase against noncognate class II tRNAs, is significantly divergent
between E.coli and yeast. This difference is thought to be due mainly to
the different composition of class II tRNAs in E.coli and yeast.
ARTICLES
Cross-species aminoacylation of tRNA with a long variable arm between Escherichia coli and Saccharomyces cerevisiae
Department of Biology, Faculty of Science, Hirosaki University, Hirosaki 036-8561, Japan. Biochemistry and Biotechnology, Faculty of.
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