Nucleic Acids Research, Vol 26, Issue 19 4426-4431, Copyright © 1998 by Oxford University Press
AK Dixon, PJ Richardson, K Lee, NP Carter and TC Freeman
The ability to relate the physiological status of individual cells to the
complement of genes they express is limited by current methodological
approaches for performing these analyses. We report here the development of
a robust and reproducible method for amplifying 3' sequences of mRNA
derived from single cells and demonstrate that the amplified cDNA, derived
from individual human lymphoblastoma cells, can be used for the expression
profiling of up to 40 different genes per cell. In addition, we show that 3
prime end amplification (TPEA) PCR can be used to enable the detection of
both high and low abundance mRNA species in samples harvested from live
neurons in rat brain slices. This procedure will facilitate the study of
complex tissue function at the cellular level.
ARTICLES
Expression profiling of single cells using 3 prime end amplification (TPEA) PCR
The Sanger Centre, The Wellcome Genome Campus, Hinxton, Cambridge CB10 1SA, UK,Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QJ, UK.
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