Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (449K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (25)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Conrad, C.
Right arrow Articles by Klug, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Conrad, C.
Right arrow Articles by Klug, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 26, Issue 19 4446-4453, Copyright © 1998 by Oxford University Press

ARTICLES

Different cleavage specificities of RNases III from Rhodobacter capsulatus and Escherichia coli

C Conrad, R Rauhut and G Klug
Institut fur Mikro- und Molekularbiologie der Justus-Liebig-Universitat Giessen, Frankfurter Strasse 107,35392 Giessen, Germany.

23S rRNA in Rhodobacter capsulatus shows endoribonuclease III (RNase III)-dependent fragmentation in vivo at a unique extra stem-loop extending from position 1271 to 1331. RNase III is a double strand (ds)- specific endoribonuclease. This substrate preference is mediated by a double-stranded RNA binding domain (dsRBD) within the protein. Although a certain degree of double strandedness is a prerequisite, the question arises what structural features exactly make this extra stem-loop an RNase III cleavage site, distinguishing it from the plethora of stem- loops in 23S rRNA? We used RNase III purified from R.capsulatus and Escherichia coli, respectively, together with well known substrates for E.coli RNase III and RNA substrates derived from the special cleavage site in R.capsulatus 23S rRNA to study the interaction between the Rhodobacter enzyme and the fragmentation site. Although both enzymes are very similar in their amino acid sequence, they exhibit significant differences in binding and cleavage of these in vitro substrates.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 RNAHome page
M. Amblar, A. Barbas, P. Gomez-Puertas, and C. M. Arraiano
The role of the S1 domain in exoribonucleolytic activity: Substrate specificity and multimerization
RNA, March 1, 2007; 13(3): 317 - 327.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. I. Purusharth, F. Klein, S. Sulthana, S. Jager, M. V. Jagannadham, E. Evguenieva-Hackenberg, M. K. Ray, and G. Klug
Exoribonuclease R Interacts with Endoribonuclease E and an RNA Helicase in the Psychrotrophic Bacterium Pseudomonas syringae Lz4W
J. Biol. Chem., April 15, 2005; 280(15): 14572 - 14578.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
D. Zaporojets, S. French, and C. L. Squires
Products Transcribed from Rearranged rrn Genes of Escherichia coli Can Assemble To Form Functional Ribosomes
J. Bacteriol., December 1, 2003; 185(23): 6921 - 6927.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Evguenieva-Hackenberg, E. Schiltz, and G. Klug
Dehydrogenases from All Three Domains of Life Cleave RNA
J. Biol. Chem., November 22, 2002; 277(48): 46145 - 46150.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
F. Klein, R. Samorski, G. Klug, and E. Evguenieva-Hackenberg
Atypical Processing in Domain III of 23S rRNA of Rhizobium leguminosarum ATCC 10004T at a Position Homologous to an rRNA Fragmentation Site in Protozoa
J. Bacteriol., June 15, 2002; 184(12): 3176 - 3185.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
B. Fournier, Q. C. Truong-Bolduc, X. Zhang, and D. C. Hooper
A Mutation in the 5' Untranslated Region Increases Stability of norA mRNA, Encoding a Multidrug Resistance Transporter of Staphylococcus aureus
J. Bacteriol., April 1, 2001; 183(7): 2367 - 2371.
[Abstract] [Full Text]

Home page
 J. Bacteriol.Home page
E. Evguenieva-Hackenberg and G. Klug
RNase III Processing of Intervening Sequences Found in Helix 9 of 23S rRNA in the Alpha Subclass of Proteobacteria
J. Bacteriol., September 1, 2000; 182(17): 4719 - 4729.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.