Nucleic Acids Research, Vol 26, Issue 2 415-419, Copyright © 1998 by Oxford University Press
Y Tozawa, K Tanaka, H Takahashi and K Wakasa
A full-length cDNA encoding a putative sigma factor for a plastid RNA
polymerase was isolated from the higher plant Oryza sativa . The nucleotide
sequence of the corresponding nuclear gene, named Os-sigA ( O.sativa sigma
A), predicts a polypeptide of 519 amino acids that contains a putative
plastid-targeting sequence in its N-terminal region. The predicted mature
protein shows extensive sequence homology to bacterial sigma factors,
encompassing the conserved regions 1.2, 2.1, 2.2, 2.3, 2.4, 3, 4.1 and 4.2
implicated in binding to -10 promoter elements, promoter melting and
interaction with the core RNA polymerase enzyme. RNA blot analysis revealed
that the abundance of Os- sigA transcripts was markedly greater in green
shoots than in roots or in dark-grown etiolated shoots of rice seedlings.
Furthermore, exposure of dark-grown etiolated seedlings to light resulted
in a rapid increase in the amount of Os-sigA mRNA in the shoot. These
observations suggest that regulation of expression of the nuclear gene for
this putative plastid RNA polymerase sigmafactor by light contributes to
light- dependent transcriptional regulation of plastid genes.
ARTICLES
Nuclear encoding of a plastid sigma factor in rice and its tissue- and light-dependent expression
National Agriculture Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305, Japan.
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