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Nucleic Acids Research, Vol 26, Issue 2 452-455, Copyright © 1998 by Oxford University Press

ARTICLES

Simple miniaturized gel system for DNA sequence analysis

A Stein, SA Hill, Z Cheng and M Bina
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA. astein@bilbo.bio.purdue.edu

A simple miniaturized gel system suitable for DNA sequencing is described. Small ultrathin polyacrylamide gels are cast, eight or more at a time, using standard microscope slides. Gels, ready to use, can be stored for approximately 2 weeks. Gels are run horizontally in a standard mini-agarose gel apparatus. Typical run times are 6-8 min. A novel sample loading system permits volumes of standard sequencing reactions as small as 0.1 microl to be analyzed. Sequencing ladders were visualized using 35S-labeled DNA by autoradiography and by colorimetric detection. Band resolution compares favorably with that of large gels. The methods introduced here serve as a step toward the miniaturization of DNA sequencing and are amenable to automated sample loading and detection.
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