Nucleic Acids Research, Vol 26, Issue 2 505-511, Copyright © 1998 by Oxford University Press
T Gross, K Richert, C Mierke, M Lutzelberger and NF Kaufer
The SR protein family is involved in constitutive and regulated pre- mRNA
splicing and has been found to be evolutionarily conserved in metazoan
organisms. In contrast, the genome of the unicellular yeast Saccharomyces
cerevisiae does not contain genes encoding typical SR proteins. The
mammalian SR proteins consist of one or two characteristic RNA binding
domains (RBD), containing the signature sequences RDAEDA and SWQDLKD
respectively, and a RS (arginine/serine- rich) domain which gave the family
its name. We have now cloned from the fission yeast Schizosaccharomyces
pombe the gene srp1. This gene is the first yeast gene encoding a protein
with typical features of mammalian SR protein family members. The gene is
not essential for growth. We show that overexpression of the RNA binding
domain inhibits pre-mRNA splicing and that the highly conserved sequence
RDAEDA in the RBD is involved. Overexpression of Srp1 containing mutations
in the RS domain also inhibits pre-mRNA splicing activity. Furthermore, we
show that overexpression of Srp1 and overexpression of the mammalian SR
splicing factor ASF/SF2 suppress the pre-mRNA splicing defect of the
temperature-sensitive prp4-73 allele. prp4 encodes a protein kinase
involved in pre-mRNA splicing. These findings are consistent with the
notion that Srp1 plays a role in the splicing process.
ARTICLES
Identification and characterization of srp1, a gene of fission yeast encoding a RNA binding domain and a RS domain typical of SR splicing factors
Institut fur Genetik-Biozentrum, Technische Universitat Braunschweig, 38106 Braunschweig, Germany.
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