Nucleic Acids Research, Vol 26, Issue 2 532-536, Copyright © 1998 by Oxford University Press
CS Hinkley, MA Blasco, WD Funk, J Feng, B Villeponteau, CW Greider and W Herr
Telomerase is a ribonucleoprotein enzyme with an essential RNA component.
Embedded within the telomerase RNA is a template sequence for telomere
synthesis. We have characterized the structure of the 5' regions of the
human and mouse telomerase-RNA genes, and have found a striking difference
in the location of the template sequence: Whereas the 5'-end of the human
telomerase RNA lies 45 nt from the telomerase- RNA template sequence, the
5'-end of the mouse telomerase RNA lies just 2 nt from the telomerase-RNA
template sequence. Analysis of genomic sequences flanking the 5'-end of the
human and mouse telomerase RNA- coding sequences reveals similar
promoter-element arrangements typical of mRNA-type promoters: a TATA
box-like element and an upstream region containing a consensus CCAAT box.
This putative promoter structure contrasts with that of the ciliate
telomerase-RNA genes whose structure resembles RNA polymerase III U6 small
nuclear RNA (snRNA) promoters. These and other comparisons suggest that,
during evolution, both the RNA-polymerase specificity of telomerase
RNA-gene promoters and, more recently, the position of the template
sequence in the telomerase RNA changed.
ARTICLES
The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence
Cold Spring Harbor Laboratory, 1 Bungtown Road, PO Box 100, Cold Spring Harbor, NY 11724, USA.
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