Nucleic Acids Research, Vol 26, Issue 20 4582-4587, Copyright © 1998 by Oxford University Press
K Fujikawa, H Kamiya and H Kasai
The mutational properties of 5-formyl-2'-deoxyuridine 5'-triphosphate
(5-CHO-dUTP) and 5-hydroxy-2'-deoxycytidine 5'-triphosphate (5-OH- dCTP),
the major oxidatively damaged pyrimidine nucleotides derived from dTTP and
dCTP, respectively, were analyzed by an in vivo assay. 5- CHO-dUTP and
5-OH-dCTP were directly incorporated into Escherichia coli , and their
mutagenicities were evaluated by the chromosomal lacI forward mutation
assay. The mutation frequencies increased, depending on the dose of these
damaged nucleotides, indicating that these nucleotides were incorporated
into E.coli and acted as mutagens in vivo . The mutagenicities of
5-CHO-dUTP and 5-OH-dCTP were comparable to that of
8-hydroxy-2'-deoxyguanosine 5'-triphosphate, a major form of dGTP oxidative
damage. 5-CHO-dUTP induced G.C to A.T, A.T to G.C and G.C to T.A mutations,
and 5-OH-dCTP elicited G.C to A.T, A.T to C.G and G.C to T.A mutations.
ARTICLES
The mutations induced by oxidatively damaged nucleotides, 5-formyl-dUTP and 5-hydroxy-dCTP,in Escherichia coli
Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
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