Nucleic Acids Research, Vol 26, Issue 20 4676-4687, Copyright © 1998 by Oxford University Press
KA Sparks and CL Dieckmann
Several yeast genes produce multiple transcripts with different 3'- ends.
Of these, four genes are known to produce truncated transcripts that end
within the coding sequence of longer transcripts: CBP1 , AEP2 / ATP13 ,
RNA14 and SIR1 . It has been shown that the level of the truncated CBP1
transcript increases during the switch to respiratory growth while that of
the full-length transcript decreases. To determine whether this phenomenon
is unique to CBP1 , northern analysis was used to determine whether the
levels of other truncated transcripts are regulated similarly by carbon
source. The levels of the shortest transcripts of AEP2 / ATP13 and RNA14
increased during respiration while the shortest SIR1 transcript remained
constant. However, two longer SIR1 transcripts were regulated reciprocally
by carbon source. Mapping the 3'-ends of each transcript by sequencing
partial cDNA clones revealed multiple 3'-ends for each transcript.
Examination of the sequences surrounding the 3'-ends of the induced
transcripts failed to identify a consensus sequence but did reveal weak
putative 3'-end formation signals in all of the transcripts. Similarly, no
consensus sequence was found when the sequences surrounding the 3'-ends of
the longest transcripts were compared, but again weak putative 3'-end
formation signals were identified. These data are suggestive of carbon
source regulation of alternative poly(A) site choice in yeast.
ARTICLES
Regulation of poly(A) site choice of several yeast mRNAs
Department of Biochemistry and Department Molecular and Cellular Biology, Life Sciences South Room 454, University of Arizona, Tucson, AZ 85721, USA.
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