Nucleic Acids Research, Vol 26, Issue 21 4874-4879, Copyright © 1998 by Oxford University Press
P Sriraman, D Silhavy and P Maliga
The existence of a phage-type plastid transcription machinery (NEP),
related to the mitochondrial RNA polymerase, has been recognized only
recently. Here we report the cis sequences required for transcription
initiation by the phage-type enzyme. The promoter chosen for the study,
PclpP-53, is well expressed in tobacco leaves, unlike most NEP promoters.
Promoter definition was carried out in vivo , in transplastomic tobacco
plants expressing a uidA reporter gene from PclpP-53 promoter derivatives.
We report here that sequences from -5 to +25 (relative to the transcription
initiation site) are sufficient to support specific transcription
initiation. Requirement of sequences downstream of the transcription
initiation site contrasts with mitochondrial promoters, which have
conserved sequences predominantly upstream. The promoter defined here is
conserved in liverworts and conifers, indicating that the phage-type
transcription machinery appeared in plastids early on during the evolution
of land plants. The PclpP-53 promoter sequences are present in rice but do
not function, suggesting that PclpP-53 recognition specificity is absent in
some monocots.
ARTICLES
The phage-type PclpP-53 plastid promoter comprises sequences downstream of the transcription initiation site
Waksman Institute, Rutgers University, 190 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA.
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