Nucleic Acids Research, Vol 26, Issue 22 5086-5092, Copyright © 1998 by Oxford University Press
K Kobayashi, S Kanno, B Smit, GT van der Horst, M Takao and A Yasui
We isolated and characterized mouse photolyase-like genes, mCRY1 (mPHLL1)
and mCRY2 (mPHLL2), which belong to the photolyase family including plant
blue-light receptors. The mCRY1 and mCRY2 genes are located on chromosome
10C and 2E, respectively, and are expressed in all mouse organs examined.
We raised antibodies specific against each gene product using its
C-terminal sequence, which differs completely between the genes.
Immunofluorescent staining of cultured mouse cells revealed that mCRY1 is
localized in mitochondria whereas mCRY2 was found mainly in the nucleus.
The subcellular distribution of CRY proteins was confirmed by immunoblot
analysis of fractionated mouse liver cell extracts. Using green fluorescent
protein fused peptides we showed that the C-terminal region of the mouse
CRY2 protein contains a unique nuclear localization signal, which is absent
in the CRY1 protein. The N-terminal region of CRY1 was shown to contain the
mitochondrial transport signal. Recombinant as well as native CRY1 proteins
from mouse and human cells showed a tight binding activity to DNA
Sepharose, while CRY2 protein did not bind to DNA Sepharose at all under
the same condition as CRY1. The different cellular localization and DNA
binding properties of the mammalian photolyase homologs suggest that
despite the similarity in the sequence the two proteins have distinct
function(s).
ARTICLES
Characterization of photolyase/blue-light receptor homologs in mouse and human cells
Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, 980 8575 Sendai, Japan.
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