Nucleic Acids Research, Vol 26, Issue 22 5176-5181, Copyright © 1998 by Oxford University Press
GJ van Eldik, K Litiere, JJ Jacobs, M Van Montagu and M Cornelissen
Post-transcriptional gene silencing of beta-1,3 glucanase genes in the
transgenic tobacco line T17 is characterised by an increased turnover and,
as a consequence, reduced levels of gn1 transgene and endogenous beta-1,3
glucanase mRNAs. Here, additional gn1 RNAs, both larger and smaller than
the full-length messenger, are shown to accumulate in silenced plants of
the transgenic tobacco line T17. The longer-than- full-length gn1 RNAs are
the result of cryptic processing of the gn1 messenger. The small gn1 RNAs
in silenced plants correspond to distal and proximal parts of the mature
gn1 messenger. The proximal RNA products are intact at their 5' extremity,
but terminate at different positions at the 3'-end. The distal RNA products
contain a poly(A) tail and are truncated to various positions at the
5'-end. These observations indicate that degradation of the mature gn1
transcript does not start at the 5'- or 3'-end, but rather are consistent
with degradation of the gn1 transcript starting with an endonucleolytic
cleavage followed by internal exonuclease digestion. Importantly, the
truncated products are more abundant in silenced plants than in expressing
plants. This suggests, together with the previously reported
silencing-related increased gn1 mRNA turnover and the similar rates of gn1
transcription in silenced and expressing T17 plants, that the predominant
decay route for the gn1 transcripts differs between silenced and expressing
conditions.
ARTICLES
Silencing of beta-1,3-glucanase genes in tobacco correlates with an increased abundance of RNA degradation intermediates
Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie,Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Luo and Z. Chen Improperly Terminated, Unpolyadenylated mRNA of Sense Transgenes Is Targeted by RDR6-Mediated RNA Silencing in Arabidopsis PLANT CELL, March 1, 2007; 19(3): 943 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sanders, N. Lannoo, W. Maddelein, A. Depicker, M. Van Montagu, M. Cornelissen, and J. Jacobs The preferred route for the degradation of silencing target RNAs in transgenic plants depends on pre-established silencing conditions Nucleic Acids Res., June 25, 2004; 32(11): 3400 - 3409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Galvani and L. Sperling Transgene-mediated post-transcriptional gene silencing is inhibited by 3' non-coding sequences in Paramecium Nucleic Acids Res., November 1, 2001; 29(21): 4387 - 4394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Vaucheret, C. Beclin, and M. Fagard Post-transcriptional gene silencing in plants J. Cell Sci., January 9, 2001; 114(17): 3083 - 3091. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Kastenmayer and P. J. Green Novel features of the XRN-family in Arabidopsis: Evidence that AtXRN4, one of several orthologs of nuclear Xrn2p/Rat1p, functions in the cytoplasm PNAS, December 5, 2000; 97(25): 13985 - 13990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Holtorf, H. Schob, C. Kunz, R. Waldvogel, and F. Meins , Jr. Stochastic and Nonstochastic Post-Transcriptional Silencing of Chitinase and ß-1,3-Glucanase Genes Involves Increased RNA Turnover—Possible Role for Ribosome-Independent RNA Degradation PLANT CELL, March 1, 1999; 11(3): 471 - 484. [Abstract] [Full Text] [PDF]
|
|