Nucleic Acids Research, Vol 26, Issue 22 5199-5202, Copyright © 1998 by Oxford University Press
MJ Prieto Alamo, J Jurado, E Francastel and F Laval
Reactive oxygen species produce different lesions in DNA. Among them,
7,8-dihydro-8-oxoguanine (8-oxoG) is one of the major oxidative products
implicated in mutagenesis. This lesion is removed from damaged DNA by base
excision repair, and genes coding for 8-oxoG-DNA glycosylases have been
isolated from bacteria, yeast and human cells. We have isolated and
characterized the cDNA encoding the rat 8-oxoG-DNA glycosylase (rOGG1).
Expression of the cDNA in the fgp mutY Escherichia coli double mutant
allowed the purification of the untagged rOGG1 protein. It excises 8-oxoG
from DNA with a strong preference for duplex DNA containing 8-oxoG:C base
pairs. rOGG1 also acts on formamidopyrimidine (FaPy) residues, and the K m
values on 8-oxoG and FaPy residues are 18.8 and 9.7 nM, respectively. When
acting on an oligonucleotide containing an 8-oxoG residue, rOGG1 shows a
beta-lyase activity that nicks DNA 3' to the lesion. However, rOGG1 acts on
a substrate containing an apurinic site by a beta-delta elimination
reaction and proceeds through a Schiff base intermediate. Expression of
rOGG1 in E.coli fpg mutY suppresses its spontaneous mutator phenotype.
ARTICLES
Rat 7,8-dihydro-8-oxoguanine DNA glycosylase: substrate specificity, kinetics and cleavagemechanism at an apurinic site
Unite 347 INSERM, 80 Rue du General Leclerc, 94276 Le Kremlin Bicetre Cedex, France.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A.-K. Olsen, N. Duale, M. Bjoras, C. T. Larsen, R. Wiger, J. A. Holme, E. C. Seeberg, and G. Brunborg Limited repair of 8-hydroxy-7,8-dihydroguanine residues in human testicular cells Nucleic Acids Res., February 15, 2003; 31(4): 1351 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Morland, V. Rolseth, L. Luna, T. Rognes, M. Bjoras, and E. Seeberg Human DNA glycosylases of the bacterial Fpg/MutM superfamily: an alternative pathway for the repair of 8-oxoguanine and other oxidation products in DNA Nucleic Acids Res., November 15, 2002; 30(22): 4926 - 4936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Navasumrit, G. P. Margison, and P. J. O'Connor ETHANOL MODULATES RAT HEPATIC DNA REPAIR FUNCTIONS Alcohol Alcohol., September 1, 2001; 36(5): 369 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gogos and N. D. Clarke Characterization of an 8-Oxoguanine DNA Glycosylase from Methanococcus jannaschii J. Biol. Chem., October 22, 1999; 274(43): 30447 - 30450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. O. Zharkov, T. A. Rosenquist, S. E. Gerchman, and A. P. Grollman Substrate Specificity and Reaction Mechanism of Murine 8-Oxoguanine-DNA Glycosylase J. Biol. Chem., September 8, 2000; 275(37): 28607 - 28617. [Abstract] [Full Text] [PDF]
|
|