Nucleic Acids Research, Vol 26, Issue 23 5365-5371, Copyright © 1998 by Oxford University Press
I Dionne and RJ Wellinger
During telomere replication in yeast, chromosome ends acquire a long
single-stranded extension of the strand making the 3' end. Previous work
showed that these 3' tails are generated late in S-phase, when conventional
replication is virtually complete. In addition, the extensions were also
observed in cells that lacked telomerase. Therefore, a model was proposed
that predicted an activity that recessed the 5' ends at yeast telomeres
after conventional replication was complete. Here, we demonstrate that this
processing activity is dependent on the passage of a replication fork
through yeast telomeres. A non-replicating linear plasmid with telomeres at
each end does not acquire single-stranded extensions, while an identical
construct containing an origin of replication does. Thus, the processing
activity could be associated with the enzymes at the replication fork
itself, or the passage of the fork through the telomeric sequences allows a
transient access for the activity to the telomeres. We therefore propose
that there is a mechanistic link between the conventional replication
machinery and telomere maintenance.
ARTICLES
Processing of telomeric DNA ends requires the passage of a replication fork
Departement de Microbiologie et Infectiologie, Faculte de Medecine, Universite de Sherbrooke, 3001 12eme Avenue Nord, Sherbrooke, Quebec J1H 5N4, Canada.
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