Nucleic Acids Research, Vol 26, Issue 24 5537-5543, Copyright © 1998 by Oxford University Press
MV Matz and SA Lukyanov
The main goal of this review is to provide some guide-lines for choosing a
proper method when searching for differentially expressed transcripts. The
choice of an approach should depend upon particular features of the
experiment and project requirements. We outline some general considerations
to help decide between differential display- related techniques and
subtractive hybridization. While discussing advantages and problems of
differential display, special attention is given to several recently
developed methods based on alternative principles of fingerprint
production, which provide a possibility for complete systematic
investigation of RNA samples.
REVIEWS
Different strategies of differential display: areas of application
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, V-437 Moscow 117871, Russia. matz@ibch.siobe.ras.ru
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