Nucleic Acids Research, Vol 26, Issue 24 5589-5595, Copyright © 1998 by Oxford University Press
LS Symington
The RAD27/RTH1 gene of Saccharomyces cerevisiae encodes a structural and
functional homolog of the 5'-3' exonuclease function of Escherichia coli
DNA polymerase I. Four alleles of RAD27 were recovered in a screen for
hyper-recombination, a phenotype also displayed by polA mutants of E.coli.
All four rad27 mutants showed similar high levels of mitotic recombination,
but varied in their growth rate at various temperatures, and sensitivity to
the DNA damaging agent methyl methane sulfonate. Dependence of viability of
rad27 strains on recombination was determined by crossing a strain
containing a null allele of RAD27 to strains containing a mutation in
either the RAD1, RAD50, RAD51, RAD52, RAD54, RAD55, RAD57, MRE11, XRS2 or
RAD59 gene. In no case were viable spore products recovered that contained
both mutations. Elimination of the non-homologous end-joining pathway did
not affect the viability of a rad27 strain. This suggests that lesions
generated in the absence of RAD27 must be processed by homologous
recombination.
ARTICLES
Homologous recombination is required for the viability of rad27 mutants
Institute of Cancer Research and Department of Microbiology, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA. lss5@columbia.edu
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