Nucleic Acids Research, Vol 26, Issue 24 5602-5608, Copyright © 1998 by Oxford University Press
A Nastos, E Pogge von Strandmann, H Weber and GU Ryffel
The tissue-specific transcription factor HNF1alpha is expressed in kidney,
liver, intestine and stomach of Xenopus. We show that the HNF1alpha gene is
transcriptionally activated at the onset of zygotic gene transcription and
that this transcription is maintained throughout development. Ectodermal
explants of blastulae (animal caps) express HNF1alpha mRNA upon stimulation
with the mesoderm inducers activin A and BMP4 as well as on overexpression
of Smad2 and Smad1, the corresponding members of the intracellular TGF-beta
signal transducers, respectively. Beside these factors that mediate their
response through serine/threonine kinase receptors, bFGF, which acts via
tyrosine kinase receptors, leads to HNF1alpha expression, too. These
embryonic inducers result in a delayed appearance of HNF1alpha mRNA,
excluding a direct activation of HNF1alpha. In contrast, the maternally
expressed nuclear receptors HNF4alpha and HNF4beta activate the initial
HNF1alpha transcription, since overexpression of HNF4 leads to a rapid
expression of HNF1alpha mRNA in animal caps. Similarly, in entire neurulae
HNF4 overexpression results in increased HNF1alpha transcription.
Therefore, we assume that the initial activation is dependent on maternal
HNF4alpha and HNF4beta transcription factors whereas HNF1alpha induction by
growth factors reflects the property of these factors to induce the
differentiation of mesodermal and entodermal cell types expressing
HNF1alpha.
ARTICLES
The embryonic expression of the tissue-specific transcription factor HNF1alpha in Xenopus: rapid activation by HNF4 and delayed induction by mesoderm inducers
Universitatsklinikum Essen, Institut fur Zellbiologie (Tumorforschung), Hufelandstrasse 55, D-45122 Essen, Germany.
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