Nucleic Acids Research, Vol 26, Issue 24 5662-5669, Copyright © 1998 by Oxford University Press
Y Habara, S Urushiyama, T Tani and Y Ohshima
In the fission yeast Schizosaccharomyces pombe, 14 prp (pre-
mRNAprocessing) mutants have been isolated to date. We cloned the prp10(+)
gene by complementation of the temperature-sensitive growth of prp10. Five
types of transcripts were found that were alternatively spliced with
respect to two possible introns located in the 5'-terminal region. Three of
them are probably functional and code for putative proteins of
approximately 1200 amino acids. Proteins highly homologous to Prp10p are
present in other organisms, one of which is a human spliceosome-associated
protein SAP155, a subunit of the splicing factor complex SF3. The
C-terminal two-thirds of Prp10p is highly conserved among species, and
contains consensus repeats for the regulatory subunit A of protein
phosphatase PP2A. A gene disruption experiment indicated that the prp10(+)
gene is essential for viability in S.pombe. Prp10p tagged with GFP is
predominantly localized in the nuclear DNA region. A series of deletions
showed that the less conserved N-terminal region of approximately 300 amino
acids in Prp10p is dispensable, although the corresponding region was
thought to play important roles in the mammalian splicing system.
ARTICLES
The fission yeast prp10(+) gene involved in pre-mRNA splicing encodes a homologue of highly conserved splicing factor, SAP155
Department of Biology, Faculty of Science, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
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