Nucleic Acids Research, Vol 26, Issue 24 5676-5683, Copyright © 1998 by Oxford University Press
W Ramos, G Liu, CN Giroux and AE Tomkinson
Here we demonstrate that the Saccharomyces cerevisiae DNA ligase activity,
which we previously designated DNA ligase II, is encoded by the genomic DNA
sequence YOR005c. Based on its homology with mammalian LIG4, this yeast
gene has been named DNL4 and the enzyme activity renamed Dnl4. In agreement
with others, we find that DNL4 is not required for vegetative growth but is
involved in the repair of DNA double-strand breaks by non-homologous end
joining. In contrast to a previous report, we find that a dnl4 null
mutation has no effect on sporulation efficiency, indicating that Dnl4 is
not required for proper meiotic chromosome behavior or subsequent
ascosporogenesis in yeast. Disruption of the DNL4 gene in one strain,
M1-2B, results in temperature-sensitive vegetative growth. At the
restrictive temperature, mutant cells progressively lose viability and
accumulate small, nucleated and non-dividing daughter cells which remain
attached to the mother cell. This novel temperature-sensitive phenotype is
complemented by retransformation with a plasmid-borne DNL4 gene. Thus, we
conclude that the abnormal growth of the dnl4 mutant strain is a synthetic
phenotype resulting from Dnl4 deficiency in combination with undetermined
genetic factors in the M1-2B strain background.
ARTICLES
Biochemical and genetic characterization of the DNA ligase encoded by Saccharomyces cerevisiae open reading frame YOR005c, a homolog of mammalian DNA ligase IV
Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center, San Antonio, TX 78245, USA.
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