Nucleic Acids Research, Vol 26, Issue 3 839-846, Copyright © 1998 by Oxford University Press
KH Emami, TW Burke and ST Smale
Sp1 is a ubiquitous activator of numerous TATA-containing and TATA-less
promoters within the human genome. This transcription factor is distinct
from several other mammalian activators because it cannot stimulate
transcription of reporter genes when ectopically expressed in Saccharomyces
cerevisiae . Here we report that in cultured cells from Drosophila
melanogaster human Sp1 efficiently activates transcription from synthetic
promoters containing TATA boxes, but not from promoters that contain an
initiator instead of a TATA box. The inability of Sp1 to activate
initiator-mediated transcription did not result from inactivity of the
consensus initiator element used for the experiments, as other initiator
functions were conserved in Drosophila cells. Interestingly, a difference
between the Drosophila and human TFIID complexes was found to be
responsible for the selective inability of Sp1 to activate
initiator-mediated transcription in Drosophila; in a complementation assay
with a TFIID-depleted HeLa cell extract both the Drosophila and human TFIID
complexes supported TATA-mediated transcription, but only the human complex
supported initiator-mediated transcription. These results suggest that a
species-specific interaction is required for activation of TATA-less
promoters by Sp1, revealing a difference in transcriptional activation
mechanisms between vertebrates and invertebrates.
ARTICLES
Sp1 activation of a TATA-less promoter requires a species-specific interaction involving transcription factor IID
Howard Hughes Medical Institute, Department of Microbiology and Immunology, UCLA School of Medicine, 675 Circle Drive South, Los Angeles, CA 90095-1662, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. G. Shah, M. J. Friedman, S. Huang, M. Roberts, X.-J. Li, and S. Li Transcriptional dysregulation of TrkA associates with neurodegeneration in spinocerebellar ataxia type 17 Hum. Mol. Genet., November 1, 2009; 18(21): 4141 - 4152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tang and E. Goldberg Homo sapiens Lactate Dehydrogenase c (Ldhc) Gene Expression in Cancer Cells Is Regulated by Transcription Factor Sp1, CREB, and CpG Island Methylation J Androl, March 1, 2009; 30(2): 157 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Quan, Z.-L. Ji, X. Wang, A. M. Tartakoff, and T. Tao Evolutionary and Transcriptional Analysis of Karyopherin {beta} Superfamily Proteins Mol. Cell. Proteomics, July 1, 2008; 7(7): 1254 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Cauvi, G. Cauvi, and K. M. Pollard Constitutive Expression of Murine Decay-Accelerating Factor 1 Is Controlled by the Transcription Factor Sp1 J. Immunol., September 15, 2006; 177(6): 3837 - 3847. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-K. Youn, S.-H. Kim, D. Y. Lee, S. H. Song, I.-Y. Chang, J.-W. Hyun, M.-H. Chung, and H. J. You Cadmium Down-regulates Human OGG1 through Suppression of Sp1 Activity J. Biol. Chem., July 1, 2005; 280(26): 25185 - 25195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Ho Sui, J. R. Mortimer, D. J. Arenillas, J. Brumm, C. J. Walsh, B. P. Kennedy, and W. W. Wasserman oPOSSUM: identification of over-represented transcription factor binding sites in co-expressed genes Nucleic Acids Res., June 2, 2005; 33(10): 3154 - 3164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-Z. Dai, F.-E. Johansen, K. M. Kolltveit, H.-C. Aasheim, Z. Dembic, F. Vartdal, and A. Spurkland Transcriptional Activation of the SH2D2A Gene Is Dependent on a Cyclic Adenosine 5'-Monophosphate-Responsive Element in the Proximal SH2D2A Promoter J. Immunol., May 15, 2004; 172(10): 6144 - 6151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Nichols, T. Itoh, F. Zolezzi, S. Hutsell, and S. Linn Basal transcriptional regulation of human damage-specific DNA-binding protein genes DDB1 and DDB2 by Sp1, E2F, N-myc and NF1 elements Nucleic Acids Res., January 15, 2003; 31(2): 562 - 569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Holmes, N. Bartle, M. Eisbacher, and B. H. Chong Cloning and Analysis of the Thrombopoietin-induced Megakaryocyte-specific Glycoprotein VI Promoter and Its Regulation by GATA-1, Fli-1, and Sp1 J. Biol. Chem., December 6, 2002; 277(50): 48333 - 48341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-J. Chew, X. Yuan, S. E. Scherer, L. Qie, F. Huang, W. P. Hayes, and V. Gallo Characterization of the Rat GRIK5 Kainate Receptor Subunit Gene Promoter and Its Intragenic Regions Involved in Neural Cell Specificity J. Biol. Chem., November 2, 2001; 276(45): 42162 - 42171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. G. Opitz and A. K. Rustgi Interaction between Sp1 and Cell Cycle Regulatory Proteins Is Important in Transactivation of a Differentiation-related Gene Cancer Res., June 1, 2000; 60(11): 2825 - 2830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. K. Ruf and J. Sample Repression of Epstein-Barr Virus EBNA-1 Gene Transcription by pRb during Restricted Latency J. Virol., October 1, 1999; 73(10): 7943 - 7951. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bernard, H. Goudonnet, Y. Artur, B. Desvergne, and W. Wahli Activation of the Mouse TATA-less and Human TATA-Containing UDP-Glucuronosyltransferase 1A1 Promoters by Hepatocyte Nuclear Factor 1 Mol. Pharmacol., September 1, 1999; 56(3): 526 - 536. [Abstract] [Full Text]
|
|