Nucleic Acids Research, Vol 26, Issue 3 847-853, Copyright © 1998 by Oxford University Press
AS Neish, SF Anderson, BP Schlegel, W Wei and JD Parvin
The RNA polymerase II (Pol II) holoenzyme in yeast is an essential
transcriptional regulatory complex which has been defined by genetic and
biochemical approaches. The mammalian counterpart to this complex, however,
is less well defined. Experiments herein demonstrate that, along with Pol
II and SRB proteins, proteins associated with transcriptional regulation as
cofactors are associated with the Pol II holoenzyme. Earlier experiments
have demonstrated that the breast cancer-associated tumor suppressor BRCA1
and the CREB binding protein (CBP) were associated with the holoenzyme
complex. The protein related to CBP, the E1A-associated p300 protein, is
shown in these experiments to be associated with the holoenzyme complex as
well as the BRG1 subunit of the chromatin remodeling SWI/SNF complex.
Importantly, the Pol II holoenzyme complex does not contain some factors
previously reported as stoichiometric components of the holoenzyme complex,
most notably the proteins which function in repair of damaged DNA, such as
PCNA, RFC and RPA. The presence of the p300 coactivator and the
chromatin-modifying BRG1 protein support a role for the Pol II holoenzyme
as a key target for regulation by enhancer binding proteins.
ARTICLES
Factors associated with the mammalian RNA polymerase II holoenzyme
Division of Molecular Oncology, Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. C. Wu and Y. Zhang Minireview: Role of Protein Methylation and Demethylation in Nuclear Hormone Signaling Mol. Endocrinol., September 1, 2009; 23(9): 1323 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hou, S. Ray, C. Lee, and A. R. Brasier The STAT3 NH2-terminal Domain Stabilizes Enhanceosome Assembly by Interacting with the p300 Bromodomain J. Biol. Chem., November 7, 2008; 283(45): 30725 - 30734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. D'Alessio, I. C. G. Weaver, and M. Szyf Acetylation-Induced Transcription Is Required for Active DNA Demethylation in Methylation-Silenced Genes Mol. Cell. Biol., November 1, 2007; 27(21): 7462 - 7474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Nandiwada, W. Li, R. Zhang, and D. L. Mueller p300/Cyclic AMP-Responsive Element Binding-Binding Protein Mediates Transcriptional Coactivation by the CD28 T Cell Costimulatory Receptor J. Immunol., July 1, 2006; 177(1): 401 - 413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Horwitz, S. Sankaran, and J. D. Parvin Direct Stimulation of Transcription Initiation by BRCA1 Requires Both Its Amino and Carboxyl Termini J. Biol. Chem., March 31, 2006; 281(13): 8317 - 8320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Dryer and L. R. Covey A Novel NF-{kappa}B-Regulated Site within the Human I{gamma}1 Promoter Requires p300 for Optimal Transcriptional Activity J. Immunol., October 1, 2005; 175(7): 4499 - 4507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Srinivasan, J. A. Armstrong, R. Deuring, I. K. Dahlsveen, H. McNeill, and J. W. Tamkun The Drosophila trithorax group protein Kismet facilitates an early step in transcriptional elongation by RNA Polymerase II Development, April 1, 2005; 132(7): 1623 - 1635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Sims III, R. Belotserkovskaya, and D. Reinberg Elongation by RNA polymerase II: the short and long of it Genes & Dev., October 15, 2004; 18(20): 2437 - 2468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J. Herrera and S. J. Triezenberg VP16-Dependent Association of Chromatin-Modifying Coactivators and Underrepresentation of Histones at Immediate-Early Gene Promoters during Herpes Simplex Virus Infection J. Virol., September 15, 2004; 78(18): 9689 - 9696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Mohrmann, K. Langenberg, J. Krijgsveld, A. J. Kal, A. J. R. Heck, and C. P. Verrijzer Differential Targeting of Two Distinct SWI/SNF-Related Drosophila Chromatin-Remodeling Complexes Mol. Cell. Biol., April 15, 2004; 24(8): 3077 - 3088. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Tien, J. P. Gray, J. M. Peters, and J. P. Vanden Heuvel Comprehensive Gene Expression Analysis of Peroxisome Proliferator-treated Immortalized Hepatocytes: Identification of Peroxisome Proliferator-activated Receptor {alpha}-dependent Growth Regulatory Genes Cancer Res., September 15, 2003; 63(18): 5767 - 5780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-y. Gui and A. Dean A major role for the TATA box in recruitment of chromatin modifying complexes to a globin gene promoter PNAS, June 10, 2003; 100(12): 7009 - 7014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Corey, C. S. Weirich, I. J. Benjamin, and R. E. Kingston Localized recruitment of a chromatin-remodeling activity by an activator in vivo drives transcriptional elongation Genes & Dev., June 1, 2003; 17(11): 1392 - 1401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fujita, R. Fujii, S. Aratani, T. Amano, A. Fukamizu, and T. Nakajima Antithetic Effects of MBD2a on Gene Regulation Mol. Cell. Biol., April 15, 2003; 23(8): 2645 - 2657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Sharma, B. Li, and J. C. Reese SWI/SNF-dependent chromatin remodeling of RNR3 requires TAFIIs and the general transcription machinery Genes & Dev., February 15, 2003; 17(4): 502 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gauthier, R. Dziak, D. J. H. Kramer, D. Leishman, X. Song, J. Ho, M. Radovic, D. Bentley, and K. Yankulov The Role of the Carboxyterminal Domain of RNA Polymerase II in Regulating Origins of DNA Replication in Saccharomyces cerevisiae Genetics, November 1, 2002; 162(3): 1117 - 1129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Prochasson, C. Delouis, and O. Brison Transcriptional deregulation of the keratin 18 gene in human colon carcinoma cells results from an altered acetylation mechanism Nucleic Acids Res., August 1, 2002; 30(15): 3312 - 3322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Chiba and J. D. Parvin The BRCA1 and BARD1 Association with the RNA Polymerase II Holoenzyme Cancer Res., August 1, 2002; 62(15): 4222 - 4228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Dellaire, E. M. Makarov, JeffJ.M. Cowger, D. Longman, H. G. E. Sutherland, R. Luhrmann, J. Torchia, and W. A. Bickmore Mammalian PRP4 Kinase Copurifies and Interacts with Components of Both the U5 snRNP and the N-CoR Deacetylase Complexes Mol. Cell. Biol., July 15, 2002; 22(14): 5141 - 5156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Hu and R. Li JunB potentiates function of BRCA1 activation domain 1 (AD1) through a coiled-coil-mediated interaction Genes & Dev., June 15, 2002; 16(12): 1509 - 1517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Livengood, K. E. S. Scoggin, K. Van Orden, S. J. McBryant, R. S. Edayathumangalam, P. J. Laybourn, and J. K. Nyborg p53 Transcriptional Activity Is Mediated through the SRC1-interacting Domain of CBP/p300 J. Biol. Chem., March 8, 2002; 277(11): 9054 - 9061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Georges, W. L. Kraus, K. Luger, J. K. Nyborg, and P. J. Laybourn p300-Mediated Tax Transactivation from Recombinant Chromatin: Histone Tail Deletion Mimics Coactivator Function Mol. Cell. Biol., January 1, 2002; 22(1): 127 - 137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Ye, Y.-F. Hu, H. Zhong, A. C. Nye, A. S. Belmont, and R. Li BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations J. Cell Biol., December 10, 2001; 155(6): 911 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Jenkins and C. A. Spencer RNA Polymerase II Holoenzyme Modifications Accompany Transcription Reprogramming in Herpes Simplex Virus Type 1-Infected Cells J. Virol., October 15, 2001; 75(20): 9872 - 9884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Chiba and J. D. Parvin Redistribution of BRCA1 among Four Different Protein Complexes following Replication Blockage J. Biol. Chem., October 12, 2001; 276(42): 38549 - 38554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K. Sullivan, C. S. Weirich, J. R. Guyon, S. Sif, and R. E. Kingston Transcriptional Activation Domains of Human Heat Shock Factor 1 Recruit Human SWI/SNF Mol. Cell. Biol., September 1, 2001; 21(17): 5826 - 5837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aratani, R. Fujii, T. Oishi, H. Fujita, T. Amano, T. Ohshima, M. Hagiwara, A. Fukamizu, and T. Nakajima Dual Roles of RNA Helicase A in CREB-Dependent Transcription Mol. Cell. Biol., July 15, 2001; 21(14): 4460 - 4469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wang, G. T. Cantin, J. L. Stevens, and A. J. Berk Characterization of Mediator Complexes from HeLa Cell Nuclear Extract Mol. Cell. Biol., July 15, 2001; 21(14): 4604 - 4613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Huber, T. W. Yang, C. J. Sarkisian, S. R. Master, C.-X. Deng, and L. A. Chodosh Impaired DNA Damage Response in Cells Expressing an Exon 11-Deleted Murine Brca1 Variant That Localizes to Nuclear Foci Mol. Cell. Biol., June 15, 2001; 21(12): 4005 - 4015. [Abstract] [Full Text]
|
|
|
|
|
|
J. D. Parvin BRCA1 at a branch point PNAS, May 22, 2001; 98(11): 5952 - 5954. [Full Text] [PDF]
|
|
|
|
|
|
A. von Mikecz, S. Zhang, M. Montminy, E. M. Tan, and P. Hemmerich Creb-Binding Protein (Cbp/P300) and RNA Polymerase II Colocalize in Transcriptionally Active Domains in the Nucleus J. Cell Biol., July 10, 2000; 150(1): 265 - 274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Kumar, K. M. McBride, B. K. Weaver, C. Dingwall, and N. C. Reich Regulated Nuclear-Cytoplasmic Localization of Interferon Regulatory Factor 3, a Subunit of Double-Stranded RNA-Activated Factor 1 Mol. Cell. Biol., June 1, 2000; 20(11): 4159 - 4168. [Abstract] [Full Text]
|
|
|
|
|
|
M. N. Szentirmay and M. Sawadogo SURVEY AND SUMMARY: Spatial organization of RNA polymerase II transcription in the nucleus Nucleic Acids Res., May 15, 2000; 28(10): 2019 - 2025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Chiba, Z. Suldan, L. P. Freedman, and J. D. Parvin Binding of Liganded Vitamin D Receptor to the Vitamin D Receptor Interacting Protein Coactivator Complex Induces Interaction with RNA Polymerase II Holoenzyme J. Biol. Chem., April 6, 2000; 275(15): 10719 - 10722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Vignali, A. H. Hassan, K. E. Neely, and J. L. Workman ATP-Dependent Chromatin-Remodeling Complexes Mol. Cell. Biol., March 15, 2000; 20(6): 1899 - 1910. [Full Text]
|
|
|
|
 |
|
 G. A. Blobel CREB-binding protein and p300: molecular integrators of hematopoietic transcription Blood, February 1, 2000; 95(3): 745 - 755. [Full Text] [PDF]
|
|
|
|
|
|
L. Wang, S. R. Grossman, and E. Kieff Epstein-Barr virus nuclear protein 2 interacts with p300, CBP, and PCAF histone acetyltransferases in activation of the LMP1 promoter PNAS, January 4, 2000; 97(1): 430 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tudor, P. J. Murray, C. Onufryk, R. Jaenisch, and R. A. Young Ubiquitous expression and embryonic requirement for RNA polymerase II coactivator subunit Srb7 in mice Genes & Dev., September 15, 1999; 13(18): 2365 - 2368. [Abstract] [Full Text]
|
|
|
|
|
|
N. Yudkovsky, C. Logie, S. Hahn, and C. L. Peterson Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators Genes & Dev., September 15, 1999; 13(18): 2369 - 2374. [Abstract] [Full Text]
|
|
|
|
|
|
S. Y. Wu, M. C. Thomas, S. Y. Hou, V. Likhite, and C. M. Chiang Isolation of Mouse TFIID and Functional Characterization of TBP and TFIID in Mediating Estrogen Receptor and Chromatin Transcription J. Biol. Chem., August 13, 1999; 274(33): 23480 - 23490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kimura, Y. Tao, R. G. Roeder, and P. R. Cook Quantitation of RNA Polymerase II and Its Transcription Factors in an HeLa Cell: Little Soluble Holoenzyme but Significant Amounts of Polymerases Attached to the Nuclear Substructure Mol. Cell. Biol., August 1, 1999; 19(8): 5383 - 5392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sune and M. A. Garcia-Blanco Transcriptional Cofactor CA150 Regulates RNA Polymerase II Elongation in a TATA-Box-Dependent Manner Mol. Cell. Biol., July 1, 1999; 19(7): 4719 - 4728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ellwood, W. Huang, R. Johnson, and M. Carey Multiple Layers of Cooperativity Regulate Enhanceosome-Responsive RNA Polymerase II Transcription Complex Assembly Mol. Cell. Biol., April 1, 1999; 19(4): 2613 - 2623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Hu, Z. L. Hao, and R. Li Chromatin remodeling and activation of chromosomal DNA replication by an acidic transcriptional activation domain from BRCA1 Genes & Dev., March 15, 1999; 13(6): 637 - 642. [Abstract] [Full Text]
|
|
|
|
|
|
D. T. Haile and J. D. Parvin Activation of Transcription in Vitro by the BRCA1 Carboxyl-terminal Domain J. Biol. Chem., January 22, 1999; 274(4): 2113 - 2117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. C. P. Holstege and R. A. Young Transcriptional regulation: Contending with complexity PNAS, January 5, 1999; 96(1): 2 - 4. [Full Text] [PDF]
|
|
|
|
|
|
V. E. Myer and R. A. Young RNA Polymerase II Holoenzymes and Subcomplexes J. Biol. Chem., October 23, 1998; 273(43): 27757 - 27760. [Full Text] [PDF]
|
|
|
|
|
|
X.-B. Qiu, Y.-L. Lin, K. C. Thome, P. Pian, B. P. Schlegel, S. Weremowicz, J. D. Parvin, and A. Dutta An Eukaryotic RuvB-like Protein (RUVBL1) Essential for Growth J. Biol. Chem., October 23, 1998; 273(43): 27786 - 27793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. K. Kim, T. H. Kim, and T. Maniatis Efficient recruitment of TFIIB and CBP-RNA polymerase II holoenzyme by an interferon-beta enhanceosome in vitro PNAS, October 13, 1998; 95(21): 12191 - 12196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Cho, G. Orphanides, X. Sun, X.-J. Yang, V. Ogryzko, E. Lees, Y. Nakatani, and D. Reinberg A Human RNA Polymerase II Complex Containing Factors That Modify Chromatin Structure Mol. Cell. Biol., September 1, 1998; 18(9): 5355 - 5363. [Abstract] [Full Text]
|
|
|
|
|
|
O Papoulas, S. Beek, S. Moseley, C. McCallum, M Sarte, A Shearn, and J. Tamkun The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes Development, January 10, 1998; 125(20): 3955 - 3966. [Abstract] [PDF]
|
|
|
|
|
|
W. Zhang, D. E. Geiman, J. M. Shields, D. T. Dang, C. S. Mahatan, K. H. Kaestner, J. R. Biggs, A. S. Kraft, and V. W. Yang The Gut-enriched Kruppel-like Factor (Kruppel-like Factor 4) Mediates the Transactivating Effect of p53 on the p21WAF1/Cip1 Promoter J. Biol. Chem., June 9, 2000; 275(24): 18391 - 18398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Hu, T. Miyake, Q. Ye, and R. Li Characterization of a Novel Trans-Activation Domain of BRCA1 That Functions in Concert with the BRCA1 C-terminal (BRCT) Domain J. Biol. Chem., December 22, 2000; 275(52): 40910 - 40915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miyake, Y.-F. Hu, D. S. Yu, and R. Li A Functional Comparison of BRCA1 C-terminal Domains in Transcription Activation and Chromatin Remodeling J. Biol. Chem., December 15, 2000; 275(51): 40169 - 40173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. P. Schlegel, V. J. Green, J. A. A. Ladias, and J. D. Parvin BRCA1 interaction with RNA polymerase II reveals a role for hRPB2 and hRPB10alpha in activated transcription PNAS, March 28, 2000; 97(7): 3148 - 3153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ouchi, S. W. Lee, M. Ouchi, S. A. Aaronson, and C. M. Horvath Collaboration of signal transducer and activator of transcription 1 (STAT1) and BRCA1 in differential regulation of IFN-gamma target genes PNAS, May 9, 2000; 97(10): 5208 - 5213. [Abstract] [Full Text] [PDF]
|
|