Nucleic Acids Research, Vol 26, Issue 4 1002-1009, Copyright © 1998 by Oxford University Press
Y Bu and MC Schmidt
We analyzed the effects of site-directed mutations in the SUC2 promoter of
Saccharomyces cerevisiae. Analyses were performed in wild-type as well as
mig1 and tup1 mutant strains after the promoter mutants were reintroduced
into the native SUC2 locus on the left arm of chromosome IX. Mutation of
the two GC boxes revealed that these elements play two distinct roles: they
are, as expected, required for Mig1-mediated repression but they are also
necessary for activation of the SUC2 promoter in response to glucose
limitation. The individual GC boxes are functionally redundant with regard
to Mig1-mediated repression, however, only the upstream GC box is essential
for high level expression of SUC2. Microccocal nuclease sensitivity of the
SUC2 promoter in derepressed cells was reduced in the GC box mutant
promoters, particularly in the vicinity of the TATA box. The difference in
nuclease sensitivity between wild-type and GC box mutant promoters was not
evident in tup1- cells. The formation of nuclease-resistant chromatin does
not require the GC boxes, indicating that other cis- acting elements can
serve to recruit the Ssn6-Tup1 co-repressor complex to the SUC2 promoter.
ARTICLES
Identification of cis-acting elements in the SUC2 promoter of Saccharomyces cerevisiae required for activation of transcription
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
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