Nucleic Acids Research, Vol 26, Issue 4 896-902, Copyright © 1998 by Oxford University Press
Z Liu, M Reches, I Groisman and H Engelberg-Kulka
The UGA codon, usually a stop codon, can also direct the incorporation into
a protein of the modified amino acid selenocysteine. This UGA decoding
process requires a cis -acting mRNA element called 'selenocysteine
insertion sequence' (SECIS) that can form a stem-loop structure. In
Escherichia coli the SECIS of the selenoprotein formate dehydrogenase
(FdhH) mRNA has been previously described to consist of at least 40
nucleotides following the UGA codon. Here we determined the nature of the
minimal SECIS required for the in vivo UGA-directed selenocysteine
incorporation in E.coli . Our study is based on extensive mutational
analysis of the fdhF SECIS DNA located in a lac' Z fusion. We found that
the whole stem-loop RNA structure of the E.coli fdhF SECIS previously
described is not required for the UGA-directed selenocysteine incorporation
in vivo . Rather, only its upper stem-loop structure of 17 nucleotides is
necessary on the condition that it is located in a proper distance (11
nucleotides) from the UGA codon. Based on these observations, we present a
new model for the minimal E.coli SECIS.
ARTICLES
The nature of the minimal 'selenocysteine insertion sequence' (SECIS) in Escherichia coli
Department of Molecular Biology, Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
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