Nucleic Acids Research, Vol 26, Issue 4 954-960, Copyright © 1998 by Oxford University Press
ES Poole, LL Major, SA Mannering and WP Tate
The observations that the Escherichia coli release factor 2 (RF2)
crosslinks with the base following the stop codon (+4 N), and that the
identity of this base strongly influences the decoding efficiency of stop
signals, stimulated us to determine whether there was a more extended
termination signal for RF2 recognition. Analysis of the 3' contexts of the
1248 genes in the E.coli genome terminating with UGA showed a strong bias
for U in the +4 position and a general bias for A and against C in most
positions to +10, consistent with the concept of an extended sequence
element. Site-directed crosslinking occurred to RF2 from a thio-U sited at
the +4, +5 and +6 bases following the UGA stop codon but not beyond (+7 to
+10). Varying the +4 to +6 bases modulated the strength of the crosslink
from the +1 invariant U to RF2. A strong selection bias for particular
bases in the +4 to +6 positions of certain E. coli UGANNN termination sites
correlated in some cases with crosslinking efficiency to RF2 and in vivo
termination signal strength. These data suggest that RF2 may recognise at
least a hexanucleotide UGA-containing sequence and that particular base
combinations within this sequence influence termination signal decoding
efficiency.
ARTICLES
Translational termination in Escherichia coli: three bases following the stop codon crosslink to release factor 2 and affect the decoding efficiency of UGA-containing signals
Department of Biochemistry and Centre for Gene Research, University of Otago, PO Box 56, Dunedin, New Zealand.
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