Nucleic Acids Research, Vol 26, Issue 4 994-1001, Copyright © 1998 by Oxford University Press
CA Johnson, LP O'Neill, A Mitchell and BM Turner
The pattern of histone H4 acetylation in different genomic regions has been
investigated by immunoprecipitating oligonucleosomes from a human
lymphoblastoid cell line with antibodies to H4 acetylated at lysines 5, 8,
12 or 16. DNA from antibody-bound or unbound chromatin was assayed by slot
blotting. Pol I and pol II transcribed genes located in euchromatin were
shown to have levels of H4 acetylation at lysines 5, 8 and 12 equivalent to
those in input chromatin, but to be slightly enriched in H4 acetylated at
lysine 16. In no case did the acetylation level correlate with actual or
potential transcriptional activity. All acetylated histone H4 isoforms were
depleted in non-coding, simple repeat DNA in heterochromatin, though the
extent of depletion varied with the type of heterochromatin and with the
isoform. Two single copy genes that map within or adjacent to blocks of
paracentric heterochromatin are depleted in H4 acetylated at lysines 5, 8
and 12, but not 16. Consensus sequences of repetitive elements of the Alu
family (SINES, enriched in R bands) were associated with H4 that was more
highly acetylated at all four lysines than input chromatin, while H4
associated with Kpn I elements (LINES, enriched in G bands) was
significantly underacetylated.
ARTICLES
Distinctive patterns of histone H4 acetylation are associated with defined sequence elements within both heterochromatic and euchromatic regions of the human genome
Chromatin and Gene Expression Group, Department of Anatomy, The Medical School, University of Birmingham, Birmingham B15 2TT, UK.
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