Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (390K) Freely available
Right arrow A corrigendum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (38)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Blom, D.
Right arrow Articles by Benne, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blom, D.
Right arrow Articles by Benne, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 26, Issue 5 1205-1213, Copyright © 1998 by Oxford University Press

ARTICLES

RNA editing in the free-living bodonid Bodo saltans [published erratum appears in Nucleic Acids Res 1998 Dec 1;26(23):5539]

D Blom, A de Haan, M van den Berg, P Sloof, M Jirku, J Lukes and R Benne
Department of Biochemistry/AMC, University of Amsterdam, Academic Medical Centre, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands.

In parasitic kinetoplastid protozoa, mitochondrial (mt) mRNAs are post- transcriptionally edited by insertion and deletion of uridylate residues, the information being provided by guide (g) RNAs. In order to further explore the role and evolutionary history of this process, we searched for editing in mt RNAs of the free-living bodonid Bodo saltans. We found extensive editing in the transcript for NADH dehydrogenase (ND) subunit 5, which is unedited in trypanosomatids. In contrast, B.saltans cytochrome c oxidase (cox) subunit 2 and maxicircle unidentified reading frame (MURF) 2 RNAs display limited editing in the same regions as their trypanosomatid counterparts. A putative intramolecular cox2 gRNA and the gene for gMURF2-I directing the insertion of only one U in the 5' editing domain of MURF2 RNA, are conserved in B.saltans. This lends (further) evolutionary support to the proposed role of these sequences as gRNAs. Phylogenetic analysis showed that B.saltans is more closely related to trypanosomatids than the cryptobiids Trypanoplasma borreli and Cryptobia helicis, in line with the trypanosomatid-like cox2 and MURF2 RNA editing patterns. Nevertheless, other features like the apparent absence of a catenated mtDNA network, are shared with bodonid and cryptobiid species. ND5 RNA editing may represent yet another example of editing 'on the way out' during kinetoplastid evolution, but in view of the fact that cox2 RNA is unedited in T. borreli and C.helicis, we infer that the editing of this RNA may have arisen relatively recently. Our results provide the first examples of RNA editing in a free-living kinetoplastid, indicating that there is no direct link between U-insertion/deletion editing and a parasitic lifestyle.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Int. J. Syst. Evol. Microbiol.Home page
T. Stoeck, M. V. J. Schwarz, J. Boenigk, M. Schweikert, S. von der Heyden, and A. Behnke
Cellular identity of an 18S rRNA gene sequence clade within the class Kinetoplastea: the novel genus Actuariola gen. nov. (Neobodonida) with description of the type species Actuariola framvarensis sp. nov.
Int J Syst Evol Microbiol, November 1, 2005; 55(6): 2623 - 2635.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
W. Marande, J. Lukes, and G. Burger
Unique Mitochondrial Genome Structure in Diplonemids, the Sister Group of Kinetoplastids
Eukaryot. Cell, June 1, 2005; 4(6): 1137 - 1146.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
D. E. GOLDEN and S. L. HAJDUK
The 3'-untranslated region of cytochrome oxidase II mRNA functions in RNA editing of African trypanosomes exclusively as a cis guide RNA
RNA, January 1, 2005; 11(1): 29 - 37.
[Abstract] [Full Text] [PDF]

Home page
 Int. J. Syst. Evol. Microbiol.Home page
D. Moreira, P. Lopez-Garcia, and K. Vickerman
An updated view of kinetoplastid phylogeny using environmental sequences and a closer outgroup: proposal for a new classification of the class Kinetoplastea
Int J Syst Evol Microbiol, September 1, 2004; 54(5): 1861 - 1875.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Gaziova and J. Lukes
Mitochondrial and Nuclear Localization of Topoisomerase II in the Flagellate Bodo saltans (Kinetoplastida), a Species with Non-catenated Kinetoplast DNA
J. Biol. Chem., March 21, 2003; 278(13): 10900 - 10907.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
A. G. B. Simpson, J. Lukes, and A. J. Roger
The Evolutionary History of Kinetoplastids and Their Kinetoplasts
Mol. Biol. Evol., December 1, 2002; 19(12): 2071 - 2083.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. Lukes, D. Lys Guilbride, J. Votypka, A. Zikova, R. Benne, and P. T. Englund
Kinetoplast DNA Network: Evolution of an Improbable Structure
Eukaryot. Cell, August 1, 2002; 1(4): 495 - 502.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Simpson, O. H. Thiemann, N. J. Savill, J. D. Alfonzo, and D. A. Maslov
Evolution of RNA editing in trypanosome mitochondria
PNAS, June 20, 2000; 97(13): 6986 - 6993.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.