Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (104K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Taniguchi, H.
Right arrow Articles by Hayashi, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Taniguchi, H.
Right arrow Articles by Hayashi, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 26, Issue 6 1481-1486, Copyright © 1998 by Oxford University Press

ARTICLES

A liquid chromatography/electrospray mass spectrometric study on the post-transcriptional modification of tRNA

H Taniguchi and N Hayashi
Division of Biomedical Polymer Science, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Aichi 470-1192, Japan. htanigut@fjuita-hu.ac.jp

Liquid chromatography/electrospray mass spectrometry is one of the rapidly developing techniques with which mass of large hydrophilic polymers such as proteins and nucleic acids can be determined precisely. The technique was applied to studies on the modifications of tRNAs. Various tRNA species purified from Escherichia coli were directly injected into a capillary reversed-phase column and the desalted and concentrated tRNAs were analyzed on-line with an electrospray mass spectrometer. In some cases, small but significant differences were noted between the theoretical and observed molecular masses, suggesting that there exist still unknown modifications. Under high resolution measurements, multiple peaks corresponding to species modified to a varying extent were resolved. To study the structures in detail, the isolated tRNA species were digested with ribonuclease T1, and the resulting mixture of fragments were analyzed by the same liquid chromatography/mass spectrometry. In this way, most of the fragments were easily identified solely from their masses, and the positions where the expected and real structures differ were revealed. The results obtained showed the presence of micro-heterogeneity among tRNAs and demonstrated at the same time the power of the hyphenated technique for the structural analysis on nucleic acids.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ReproductionHome page
C Baumann, B Davies, M Peters, U Kaufmann-Reiche, M Lessl, and F Theuring
AKR1B7 (mouse vas deferens protein) is dispensable for mouse development and reproductive success
Reproduction, July 1, 2007; 134(1): 97 - 109.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
M. G. Goll, F. Kirpekar, K. A. Maggert, J. A. Yoder, C.-L. Hsieh, X. Zhang, K. G. Golic, S. E. Jacobsen, and T. H. Bestor
Methylation of tRNAAsp by the DNA Methyltransferase Homolog Dnmt2
Science, January 20, 2006; 311(5759): 395 - 398.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. F. Waas, V. de Crecy-Lagard, and P. Schimmel
Discovery of a Gene Family Critical to Wyosine Base Formation in a Subset of Phenylalanine-specific Transfer RNAs
J. Biol. Chem., November 11, 2005; 280(45): 37616 - 37622.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.