Nucleic Acids Research, Vol 26, Issue 7 1621-1627, Copyright © 1998 by Oxford University Press
WN Tawe, ML Eschbach, RD Walter and K Henkle-Duhrsen
In order to identify genes that are differentially expressed as a
consequence of oxidative stress due to paraquat we used the differential
display technique to compare mRNA expression patterns in Caenorhabditis
elegans . A C.elegans mixed stage worm population and a homogeneous larval
population were treated with 100 mM paraquat, in parallel with controls.
Induction of four cDNA fragments, designated L- 1, M-47, M-96 and M-132,
was confirmed by Northern blot analysis with RNA from stressed and
unstressed worm populations. A 40-fold increase in the steady-state mRNA
level in the larval population was observed for the L-1/M-47 gene, which
encodes the detoxification enzyme glutathione S-transferase. A potential
stress-responsive transcription factor (M-132) with C2H2-type zinc finger
motifs and an N-terminal leucine zipper domain was identified. The M-96
gene encodes a novel stress-responsive protein. Since paraquat is known to
generate superoxide radicals in vivo , the response of the C.elegans
superoxide dismutase (SOD) genes to paraquat was also investigated in this
study. The steady-state mRNA levels of the manganese-type and the
copper/zinc- type SODs increased 2-fold in the larval population in
response to paraquat, whereas mixed stage populations did not show any
apparent increase in the levels of these SOD mRNAs.
ARTICLES
Identification of stress-responsive genes in Caenorhabditis elegans using RT-PCR differential display
Department of Biochemistry, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany.
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