Nucleic Acids Research, Vol 26, Issue 7 1653-1659, Copyright © 1998 by Oxford University Press
R Cartwright, AM Dunn, PJ Simpson, CE Tambini and J Thacker
Genes from the recA/RAD51 family play essential roles in homologous
recombination in all organisms. Using sequence homologies from eukaryotic
members of this family we have identified fragments of two additional
mammalian genes with homology to RAD51. Cloning the full- length cDNAs for
both human and mouse genes showed that the sequences are highly conserved,
and that the predicted proteins have characteristic features of this gene
family. One of the novel genes (R51H2) occurs in two forms in human cDNA,
differing extensively at the 3' end, probably due to an unusual form of
alternative splicing. The new genes (R51H2 and R51H3) were mapped to human
chromosomes 14q23-24 and 17q1.2, respectively. Expression studies showed
that R51H2 is expressed at lower levels than R51H3 , but that expression of
both genes occurs at elevated levels in the testis compared with other
tissues. The combination of gene structure conservation and the transcript
expression patterns suggests that these new members of the recA/RAD51
family may also function in homologous recombination-repair pathways.
ARTICLES
Isolation of novel human and mouse genes of the recA/RAD51 recombination-repair gene family
Medical Research Council, Radiation and Genome Stability Unit, Harwell, Oxfordshire OX11 0RD, UK.
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