Nucleic Acids Research, Vol 26, Issue 9 2050-2057, Copyright © 1998 by Oxford University Press
B Schwer, X Mao and S Shuman
Current models of mRNA decay in yeast posit that 3' deadenylation precedes
enzymatic removal of the 5' cap, which then exposes the naked end to 5'
exonuclease action. Here, we analyzed gene expression in Saccharomyces
cerevisiae cells bearing conditional mutations of Ceg1 (capping enzyme), a
52 kDa protein that transfers GMP from GTP to the 5' end of mRNA to form
the GpppN cap structure. Shift of ceg1 mutants to restrictive temperature
elicited a rapid decline in the rate of protein synthesis, which correlated
with a sharp reduction in the steady-state levels of multiple individual
mRNAs. ceg1 mutations prevented the accumulation of SSA1 and SSA4 mRNAs
that were newly synthesized at the restrictive temperature. Uncapped
poly(A)+ SSA4 mRNA accumulated in cells lacking the 5' exoribonuclease
Xrn1. These findings provide genetic evidence for the long-held idea that
the cap guanylate is critical for mRNA stability. The
deadenylation-decapping- degradation pathway appears to be short-circuited
when Ceg1 is inactivated.
ARTICLES
Accelerated mRNA decay in conditional mutants of yeast mRNA capping enzyme
Microbiology Department, Cornell University Medical College, New York and Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10021, USA.
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