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Nucleic Acids Research, Vol 26, Issue 9 2058-2062, Copyright © 1998 by Oxford University Press

ARTICLES

Phosphorylation of tobacco eukaryotic translation initiation factor 4A upon pollen tube germination

RG op den Camp and C Kuhlemeier
Institute of Plant Physiology, University of Berne, Altenbergrain 21, CH-3013 Berne, Switzerland.

Eukaryotic translation initiation factor eIF-4A is a member of the DEAD box family of RNA helicases and RNA-dependent ATPases. In tobacco, eIF- 4A is encoded by a gene family with one isoform, eIF-4A8, being exclusively expressed in pollen. This pollen-specific isoform is a candidate for mediating translational control in the developing gametophyte. Here we show that eIF-4A is barely phosphorylated in mature pollen, but during pollen tube germination, two isoforms of eIF- 4A become phosphorylated. Phosphoamino acid analysis indicated phosphorylation of threonine. In order to determine whether pollen- specific eIF-4A8 is among the phosphorylated isoforms, we raised transgenic tobacco plants overexpressing eIF-4A8 containing a histidine tag. Hereby, we could show that indeed eIF-4A8 is modified through phosphorylation. The biological relevance of the phosphorylation of eIF- 4A is discussed.
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