Nucleic Acids Research, Vol 26, Issue 9 2075-2081, Copyright © 1998 by Oxford University Press
B Li, H Wing, D Lee, HC Wu and S Busby
During transcription activation at FNR-dependent promoters where the DNA
site for FNR overlaps the -35 element, a surface-exposed activating region
in the upstream subunit of the FNR dimer interacts with the C- terminal
domain of the RNA polymerase alpha subunit. Starting with a cloned fnr gene
encoding a defective FNR derivative carrying substitutions in this
activating region, we screened a library of random mutations to identify
substitutions that restored FNR activity. Activity can be restored by
substitutions at residues T118, E47 and K60. The locations of these
residues identify three separate surface- exposed regions of FNR that can
play a role in transcription activation. These three regions appear to be
analogues of Activating Region 1, Activating Region 2 and Activating Region
3 of the cyclic AMP receptor protein, CRP: our results underscore the
similarities between FNR and CRP.
ARTICLES
Transcription activation by Escherichia coli FNR protein: similarities to, and differences from, the CRP paradigm
School of Biochemistry, The University of Birmingham, Birmingham B15 2TT, UK.
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