Nucleic Acids Research, Vol 26, Issue 9 2098-2104, Copyright © 1998 by Oxford University Press
VP Iyemere, NH Davies and GG Brownlee
Hepatic nuclear factor 4 (HNF4) is a transcription factor whose expression
is crucial for mouse embryonic development, for liver- specific gene
expression and for the prevention of one form of maturity- onset diabetes
of the young. Its domain structure has been defined previously and is
similar to other members of the nuclear receptor superfamily. A repressor
domain has now been localised to a region of 14 amino acids (residues
428-441) near the C-terminus of HNF4 and is sufficient by itself to repress
the activity of the activation function 2 (AF2) domain. Multiple mutations
within this repressor domain enhance activity. Interestingly, this
repressor domain shares homology with a repressor domain in the
progesterone receptor. In a detailed mutagenesis study of the AF2 core, we
demonstrate that L 366, which is conserved in the AF2 core between HNF4 and
a number of orphan nuclear receptors, is essential for the full activity of
the AF2 domain. Furthermore, a double mutation of E 363 and L 366 suggests
that these residues might act in a cooperative manner.
ARTICLES
The activation function 2 domain of hepatic nuclear factor 4 is regulated by a short C-terminal proline-rich repressor domain
Chemical Pathology Unit, Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. W. Harries, J. M. Locke, B. Shields, N. A. Hanley, K. P. Hanley, A. Steele, P. R. Njolstad, S. Ellard, and A. T. Hattersley The Diabetic Phenotype in HNF4A Mutation Carriers Is Moderated By the Expression of HNF4A Isoforms From the P1 Promoter During Fetal Development Diabetes, June 1, 2008; 57(6): 1745 - 1752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Petrescu, R. Hertz, J. Bar-Tana, F. Schroeder, and A. B. Kier Role of Regulatory F-domain in Hepatocyte Nuclear Factor-4{alpha} Ligand Specificity J. Biol. Chem., April 29, 2005; 280(17): 16714 - 16727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Eeckhoute, P. Formstecher, and B. Laine Hepatocyte Nuclear Factor 4{alpha} enhances the Hepatocyte Nuclear Factor 1{alpha}-mediated activation of transcription Nucleic Acids Res., May 11, 2004; 32(8): 2586 - 2593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Childs and S. Goodbourn Identification of novel co-repressor molecules for Interferon Regulatory Factor-2 Nucleic Acids Res., June 15, 2003; 31(12): 3016 - 3026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Ruse Jr., M. L. Privalsky, and F. M. Sladek Competitive Cofactor Recruitment by Orphan Receptor Hepatocyte Nuclear Factor 4{alpha}1: Modulation by the F Domain Mol. Cell. Biol., March 15, 2002; 22(6): 1626 - 1638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Schrem, J. Klempnauer, and J. Borlak Liver-Enriched Transcription Factors in Liver Function and Development. Part I: The Hepatocyte Nuclear Factor Network and Liver-Specific Gene Expression Pharmacol. Rev., March 1, 2002; 54(1): 129 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. M. Sladek, M. D. Ruse Jr., L. Nepomuceno, S.-M. Huang, and M. R. Stallcup Modulation of Transcriptional Activation and Coactivator Interaction by a Splicing Variation in the F Domain of Nuclear Receptor Hepatocyte Nuclear Factor 4alpha 1 Mol. Cell. Biol., October 1, 1999; 19(10): 6509 - 6522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hosoda, K. Monzen, Y. Hiroi, T. Oka, E. Takimoto, Y. Yazaki, R. Nagai, and I. Komuro A Novel Myocyte-specific Gene Midori Promotes the Differentiation of P19CL6 Cells into Cardiomyocytes J. Biol. Chem., September 14, 2001; 276(38): 35978 - 35989. [Abstract] [Full Text] [PDF]
|
|