Nucleic Acids Research, Vol 26, Issue 9 2112-2119, Copyright © 1998 by Oxford University Press
H Heller and E Bengal
The myogenic determination factor MyoD activates the transcription of
muscle-specific genes by binding to consensus DNA sites found in the
regulatory sequences of these genes. The interaction of MyoD with the basal
transcription machinery is not known. Several activators induce
transcription by recruiting TFIID and/or TFIIB to the promoter. We asked
whether MyoD interacted functionally with TFIID and TFIIB in transcription.
We reconstituted in vitro DNA binding and transcription systems of MyoD and
basal transcription factors, and found that MyoD function in transcription
occurred during the assembly of the preinitiation complex. Interestingly,
MyoD activated transcription without affecting the binding of TFIID to the
promoter. However, TFIID or TBP dramatically stabilized the binding of MyoD
to its recognition site. MyoD and TBP interacted in solution. Deletion
analysis of MyoD suggested that interaction of MyoD with TBP is needed for
its activity in transcription. At a later stage of assembly, MyoD
stabilized the binding of TFIIB to the preinitiation complex. These
findings suggest that MyoD is involved in two steps of preinitiation;
first, TFIID stabilizes MyoD binding to its DNA recognition site and at a
later stage MyoD facilitates the association of TFIIB with the
preinitiation complex.
ARTICLES
TFIID (TBP) stabilizes the binding of MyoD to its DNA site at the promoter and MyoD facilitates the association of TFIIB with the preinitiation complex
Department of Biochemistry, Rappaport Institute for Research in the Medical Sciences, Faculty of Medicine, Technion-Israel Institute of Technology, PO Box 9649, Haifa 31096, Israel.
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