Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (364K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Amundsen, S. K.
Right arrow Articles by Smith, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Amundsen, S. K.
Right arrow Articles by Smith, G. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 26, Issue 9 2125-2131, Copyright © 1998 by Oxford University Press

ARTICLES

A stimulatory RNA associated with RecBCD enzyme

SK Amundsen, AF Taylor and GR Smith
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, A1- 162, PO Box 19024, Seattle, WA 98109, USA.

RecBCD enzyme acts in the major pathway of homologous recombination of linear DNA in Escherichia coli. The enzyme unwinds DNA and is an ATP- dependent double-strand and single-strand exonuclease and a single- strand endonuclease; it acts at Chi recombination hotspots (5'-GCTGGTGG- 3') to produce a recombinogenic single-stranded DNA 3'-end. We found that a small RNA with a unique sequence of approximately 24 nt was tightly bound to RecBCD enzyme and co-purified with it. When added to native enzyme this RNA, but not four others, increased DNA unwinding and Chi nicking activities of the enzyme. In seven similarly active enzyme preparations the molar ratio of RNA molecules to RecBCD enzyme molecules ranged from 0.2 to <0.008. These results suggest that, although this unique RNA is not an essential enzyme subunit, it has a biological role in stimulating RecBCD enzyme activity.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Microbiol. Mol. Biol. Rev.Home page
M. S. Dillingham and S. C. Kowalczykowski
RecBCD Enzyme and the Repair of Double-Stranded DNA Breaks
Microbiol. Mol. Biol. Rev., December 1, 2008; 72(4): 642 - 671.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. K. Amundsen, A. F. Taylor, and G. R. Smith
The RecD subunit of the Escherichia coli RecBCD enzyme inhibits RecA loading, homologous recombination, and DNA repair
PNAS, June 6, 2000; (2000) 130192397.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. K. Amundsen, A. F. Taylor, and G. R. Smith
The RecD subunit of the Escherichia coli RecBCD enzyme inhibits RecA loading, homologous recombination, and DNA repair
PNAS, June 20, 2000; 97(13): 7399 - 7404.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.